Clinical significance of LINC02532 in hepatitis B virus-associated hepatocellular carcinoma and its regulatory effect on tumor progression

IF 2.6 4区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY

Clinics and research in hepatology and gastroenterology Pub Date : 2024-06-19 DOI:10.1016/j.clinre.2024.102403

Wei Ding , Sujuan Xi , Kewei Gao , Danping Weng , Sheng Xu , Guoping Huang , Min Yu , Haiyan Yue , Jianguo Wang

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引用次数: 0

Abstract

Background and aim

Long non-coding RNAs (lncRNAs) play an important role in tumor progression, including in hepatocellular carcinoma (HCC) induced by hepatitis B virus (HBV). Therefore, the aim of this study was to investigate the role of LINC02532 in HCC, mainly for diagnostic prognostic value and cellular function, as well as mechanistic aspects.

Methods

Initially, GEO and VirBase databases were used to screen for aberrant lncRNAs in HBV-HCC.Then, HBV-HCC persons followed up in our center were retrospectively studied to investigate the diagnostic, prognostic value of LINC02532 in HBV-HCC. Subsequently, the role of LINC02532 in HBV-HCC was measured using cellular function assay methods and possible mechanisms were analyzed in conjunction with bioinformatic predictive science.

Results

LINC02532 was a lncRNA abnormally expressed in HBV-HCC. LINC02532 was significantly up-regulated in the expression level in HBV-HCC tissues compared with normal tissues from patients. Moreover, LINC02532 could distinguish HBV-HCC and predict the prognosis of HBV-HCC. In vitro experiments showed that LINC02532 could regulate miR-455–3p and promote the malignant characterization of HBV-HCC cells. CHEK2 was a target gene of miR-455–3p.

Conclusions

: The prognosis and diagnosis of HBV-HCC can rely on the expression of LINC02532. LINC02532 was important for further progression of HBV-HCC, by moderating miR-455–3p/CHEK2.

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LINC02532 在乙型肝炎病毒相关性肝细胞癌中的临床意义及其对肿瘤进展的调控作用

背景和目的：长非编码RNA（lncRNA）在肿瘤进展中发挥着重要作用，包括在乙型肝炎病毒（HBV）诱导的肝细胞癌（HCC）中。因此，本研究旨在研究 LINC02532 在 HCC 中的作用，主要是诊断预后价值和细胞功能以及机理方面：首先，利用GEO和VirBase数据库筛选HBV-HCC中的异常lncRNA。然后，对本中心随访的HBV-HCC患者进行回顾性研究，探讨LINC02532在HBV-HCC中的诊断和预后价值。随后，利用细胞功能检测方法测定了LINC02532在HBV-HCC中的作用，并结合生物信息预测科学分析了可能的机制：结果：LINC02532是一种在HBV-HCC中异常表达的lncRNA。与患者正常组织相比，LINC02532在HBV-HCC组织中的表达水平明显上调。此外，LINC02532 还能区分 HBV-HCC，并预测 HBV-HCC 的预后。体外实验表明，LINC02532 可以调控 miR-455-3p，促进 HBV-HCC 细胞的恶性化。CHEK2是miR-455-3p的靶基因：结论：HBV-HCC的预后和诊断取决于LINC02532的表达。LINC02532通过调节miR-455-3p/CHEK2，对HBV-HCC的进一步发展起着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinics and research in hepatology and gastroenterology GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

4.30

自引率

3.70%

发文量

198

审稿时长

42 days

期刊介绍： Clinics and Research in Hepatology and Gastroenterology publishes high-quality original research papers in the field of hepatology and gastroenterology. The editors put the accent on rapid communication of new research and clinical developments and so called "hot topic" issues. Following a clear Editorial line, besides original articles and case reports, each issue features editorials, commentaries and reviews. The journal encourages research and discussion between all those involved in the specialty on an international level. All articles are peer reviewed by international experts, the articles in press are online and indexed in the international databases (Current Contents, Pubmed, Scopus, Science Direct). Clinics and Research in Hepatology and Gastroenterology is a subscription journal (with optional open access), which allows you to publish your research without any cost to you (unless you proactively chose the open access option). Your article will be available to all researchers around the globe whose institution has a subscription to the journal.