Unleashing the potential: type I CRISPR-Cas systems in actinomycetes for genome editing

IF 10.2 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Natural Product Reports Pub Date : 2024-09-18 DOI:10.1039/d4np00010b

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引用次数: 0

Abstract

Covering: up to the end of 2023

Type I CRISPR-Cas systems are widely distributed, found in over 40% of bacteria and 80% of archaea. Among genome-sequenced actinomycetes (particularly Streptomyces spp.), 45.54% possess type I CRISPR-Cas systems. In comparison to widely used CRISPR systems like Cas9 or Cas12a, these endogenous CRISPR-Cas systems have significant advantages, including better compatibility, wide distribution, and ease of operation (since no exogenous Cas gene delivery is needed). Furthermore, type I CRISPR-Cas systems can simultaneously edit and regulate genes by adjusting the crRNA spacer length. Meanwhile, most actinomycetes are recalcitrant to genetic manipulation, hindering the discovery and engineering of natural products (NPs). The endogenous type I CRISPR-Cas systems in actinomycetes may offer a promising alternative to overcome these barriers. This review summarizes the challenges and recent advances in CRISPR-based genome engineering technologies for actinomycetes. It also presents and discusses how to establish and develop genome editing tools based on type I CRISPR-Cas systems in actinomycetes, with the aim of their future application in gene editing and the discovery of NPs in actinomycetes.

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释放潜能：放线菌中用于基因组编辑的 I 型 CRISPR-Cas 系统。

覆盖范围：截至 2023 年底I 型 CRISPR-Cas 系统分布广泛，存在于 40% 以上的细菌和 80% 的古细菌中。在基因组测序的放线菌（尤其是链霉菌属）中，45.54%拥有I型CRISPR-Cas系统。与广泛使用的CRISPR系统（如Cas9或Cas12a）相比，这些内源CRISPR-Cas系统具有显著的优势，包括兼容性更好、分布广泛、操作简便（因为不需要外源Cas基因的传递）。此外，I型CRISPR-Cas系统可以通过调整crRNA间隔长度来同时编辑和调控基因。与此同时，大多数放线菌对基因操作不敏感，阻碍了天然产物（NPs）的发现和工程化。放线菌中的内源性 I 型 CRISPR-Cas 系统可能为克服这些障碍提供了一种有前途的选择。本综述总结了放线菌基于 CRISPR 的基因组工程技术所面临的挑战和最新进展。它还介绍和讨论了如何在放线菌中建立和开发基于 I 型 CRISPR-Cas 系统的基因组编辑工具，目的是将来将其应用于放线菌的基因编辑和 NPs 的发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Natural Product Reports 化学-生化与分子生物学

CiteScore

21.20

自引率

3.40%

发文量

127

审稿时长

1.7 months

期刊介绍： Natural Product Reports (NPR) serves as a pivotal critical review journal propelling advancements in all facets of natural products research, encompassing isolation, structural and stereochemical determination, biosynthesis, biological activity, and synthesis. With a broad scope, NPR extends its influence into the wider bioinorganic, bioorganic, and chemical biology communities. Covering areas such as enzymology, nucleic acids, genetics, chemical ecology, carbohydrates, primary and secondary metabolism, and analytical techniques, the journal provides insightful articles focusing on key developments shaping the field, rather than offering exhaustive overviews of all results. NPR encourages authors to infuse their perspectives on developments, trends, and future directions, fostering a dynamic exchange of ideas within the natural products research community.