{"title":"Annual variation in yield, chemical composition, antioxidant and antibacterial activity of rockrose (Cistus ladanifer L.) essential oils","authors":"Veronika Chaloupková , Irene Mediavilla , Raquel Bados , Markéta Houdková , Johana Rondevaldová , Luis Saúl Esteban","doi":"10.1016/j.bcab.2024.103279","DOIUrl":null,"url":null,"abstract":"<div><p>The fluctuation in yield, composition, antibacterial and antioxidant activity of the essential oil (EO) of rockrose (<em>Cistus ladanifer</em> L.) obtained from two locations in central Spain was analysed and compared over the course of one year. Fifteen kilograms of <em>C. ladanifer</em>, collected monthly or fortnightly from two sites with different plant age (7 and 12 years), underwent steam-distillation. GC (FID)-MS analyses were done to determine the volatile profile of EOs. Obtained EO yields varied significantly (<em>P</em> < 0.001) throughout the year, ranging between 0.03 and 0.19% w/d.w. (±0.05) with the highest yields (>0.1%) observed from September to February. The younger population exhibited higher EO yields that the older one. Both rockrose populations displayed a high content of α-pinene (40.4–62.0%); the other predominant constituents included <em>allo</em>-aromadendrene + <em>trans</em>-pinocarveol, limonene, bornyl acetate and viridiflorol. The higher EO yields coincided with a higher content of α-pinene and a lower content of the other components. Furthermore, EOs’ antibacterial potential was tested against <em>Bacillus cereus</em>. Only a weak antibacterial potency (512–1024 μg/mL) was revealed, with stronger potency in case of older rockrose population. <em>In vitro</em> antioxidant capacity, evaluated by ORAC method, showed similar results for both populations, with its stronger potential between February and May.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878818124002639/pdfft?md5=e5c77dfd5cea1164ae83e59418e361b6&pid=1-s2.0-S1878818124002639-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biocatalysis and agricultural biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1878818124002639","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The fluctuation in yield, composition, antibacterial and antioxidant activity of the essential oil (EO) of rockrose (Cistus ladanifer L.) obtained from two locations in central Spain was analysed and compared over the course of one year. Fifteen kilograms of C. ladanifer, collected monthly or fortnightly from two sites with different plant age (7 and 12 years), underwent steam-distillation. GC (FID)-MS analyses were done to determine the volatile profile of EOs. Obtained EO yields varied significantly (P < 0.001) throughout the year, ranging between 0.03 and 0.19% w/d.w. (±0.05) with the highest yields (>0.1%) observed from September to February. The younger population exhibited higher EO yields that the older one. Both rockrose populations displayed a high content of α-pinene (40.4–62.0%); the other predominant constituents included allo-aromadendrene + trans-pinocarveol, limonene, bornyl acetate and viridiflorol. The higher EO yields coincided with a higher content of α-pinene and a lower content of the other components. Furthermore, EOs’ antibacterial potential was tested against Bacillus cereus. Only a weak antibacterial potency (512–1024 μg/mL) was revealed, with stronger potency in case of older rockrose population. In vitro antioxidant capacity, evaluated by ORAC method, showed similar results for both populations, with its stronger potential between February and May.
期刊介绍:
Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.