Long-term exposure of metamifop affects sex differentiation and reproductive system of zebrafish (Danio rerio)

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-06-15 DOI:10.1016/j.aquatox.2024.107004

Mengyu Guo , Feng Zhao , Mengna Zhang , Xiangguang Chen , Manman Duan , Yao Xie , Zhongyu Zhang , Jiazhen Jiang , Lihong Qiu

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引用次数: 0

Abstract

The extensive use of herbicide metamifop (MET) in rice fields for weeds control will inevitably lead to its entering into water environments and threaten the aquatic organisms. Previous researches have demonstrated that sublethal exposure of MET significantly affected zebrafish development. Yet the long-term toxicological impacts of MET on aquatic life remains unknown. Herein, we investigated the potential effects of MET (5 and 50 μg/L) on zebrafish during an entire life cycle. Since the expression level of male sex differentiation-related gene dmrt1 and sex hormone synthesis-related gene cyp19a1b were significantly changed after 50 μg/L MET exposure for only 7 days, indicators related to sex differentiation and reproductive system were further investigated. Results showed that the transcript of dmrt1 was inhibited, estradiol content increased and testosterone content decreased in zebrafish of both sexes after MET exposure at 45, 60 and 120 dpf. Histopathological sections showed that the proportions of mature germ cells in the gonads of male and female zebrafish (120 dpf) were significantly decreased. Moreover, males had elevated vitellogenin content while females did not after MET exposure; MET induced feminization in zebrafish, with the proportion of females significantly increased by 19.6% while that of males significantly decreased by 13.2% at 120 dpf. These results suggested that MET interfered with the expression levels of gonad development related-genes, disrupted sex hormone balance, and affected sex differentiation and reproductive system of female and male zebrafish, implying it might have potential endocrine disrupting effects after long-term exposure.

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长期接触甲胺磷会影响斑马鱼（Danio rerio）的性别分化和生殖系统

除草剂甲胺磷（MET）被广泛用于稻田除草，不可避免地会进入水环境，对水生生物造成威胁。先前的研究表明，亚致死接触 MET 会显著影响斑马鱼的发育。然而，MET 对水生生物的长期毒理影响仍然未知。在此，我们研究了 MET（5 和 50 μg/L）对斑马鱼整个生命周期的潜在影响。由于与雄性性别分化相关的基因 dmrt1 和与性激素合成相关的基因 cyp19a1b 在接触 50 μg/L MET 仅 7 天后表达水平发生了显著变化，因此我们进一步研究了与性别分化和生殖系统相关的指标。结果表明，暴露于 MET 后，45、60 和 120 dpf 的雌雄斑马鱼的 dmrt1 转录本受到抑制，雌二醇含量增加，睾酮含量减少。组织病理学切片显示，雌雄斑马鱼（120 dpf）性腺中成熟生殖细胞的比例显著下降。此外，暴露于MET后，雄性斑马鱼的卵黄素含量升高，而雌性斑马鱼则没有；MET诱导斑马鱼雌性化，在120 dpf时，雌性斑马鱼的比例显著增加了19.6%，而雄性斑马鱼的比例显著减少了13.2%。这些结果表明，MET干扰了性腺发育相关基因的表达水平，破坏了性激素平衡，影响了雌性和雄性斑马鱼的性别分化和生殖系统，意味着长期接触MET可能会产生潜在的内分泌干扰效应。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.