Nanobody and CuS Nanoflower-Au-Based Lateral Flow Immunoassay Strip to Enhance the Detection of Aflatoxin B1

Foods Pub Date : 2024-06-12 DOI:10.3390/foods13121845

Yiming Zhao, Baoshan He, Danyang Li, Leyan Gao, Wenjie Ren

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Abstract

In the realm of analysis, the lateral flow immunoassay (LFIA) is frequently utilized due to its capability to be fast and immediate. However, the biggest challenge of the LFIA is its low detection sensitivity and tolerance to matrix interference, making it impossible to enable accurate, qualitative analyses. In this study, we developed a new LFIA with higher affinity and sensitivity, based on a nanobody (G8-DIG) and CuS nanoflowers-Au (CuS NFs-Au), for the detection of aflatoxin B1 (AFB1) in maize. We synthesized the immunoprobe G8-DIG@CuS NFs-Au, stimulated the in situ development of Au nanoparticles (Au NPs) on Cu NFs by electrical displacement, and obtained Cu NFs-Au for fixing the G8-DIG. G8-DIG@CuS NFs-Au probe-based LFIAs may, in ideal circumstances, use a strip chromatography reader to accomplish sensitive quantitative detection and qualitative visualization. AFB1 has a detection range of 2.82–89.56 µg/L and a detection limit of 0.87 µg/L. When compared with an LFIA based on CuS NFs, this sensitivity is increased by 2.76 times. The practical application of this method in corn flour demonstrated a recovery rate of 81.7% to 117%. Therefore, CuS NFs-Au show great potential for detecting analytes.

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基于纳米抗体和 CuS 纳米花-金的侧向流免疫测定带可提高黄曲霉毒素 B1 的检测能力

在分析领域，侧流免疫分析法（LFIA）因其快速、即时的特点而被广泛使用。然而，横向流动免疫分析法面临的最大挑战是检测灵敏度低、易受基质干扰，因此无法进行准确的定性分析。在这项研究中，我们开发了一种基于纳米抗体（G8-DIG）和铜氧化物纳米花-金（CuS NFs-Au）的新型 LFIA，具有更高的亲和力和灵敏度，可用于检测玉米中的黄曲霉毒素 B1（AFB1）。我们合成了免疫探针 G8-DIG@CuS NFs-Au，通过电位移刺激金纳米颗粒（Au NPs）在 Cu NFs 上原位发育，得到了固定 G8-DIG 的 Cu NFs-Au。在理想情况下，基于 G8-DIG@CuS NFs-Au 探针的 LFIA 可使用条带色谱阅读器完成灵敏的定量检测和定性显像。AFB1 的检测范围为 2.82-89.56 µg/L，检测限为 0.87 µg/L。与基于 CuS NFs 的 LFIA 相比，灵敏度提高了 2.76 倍。该方法在玉米粉中的实际应用表明，其回收率为 81.7% 至 117%。因此，CuS NFs-Au 在检测分析物方面显示出巨大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Foods

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