The effects of saluang belum (Luvunga sarmentosa) root infusion on sperm motility, viability, and testicle histology in mice (Mus musculus)

Abstract

Background: Men's infertility is frequently caused by a decrease in sperm quality. Herbal medicine, which is relatively cheap and easy to obtain, can be used to treat this condition. People in Central Borneo frequently consume boiled Saluang Belum root (Luvunga sarmentosa). L. sarmentosa root has the potential to improve sperm quality.      Objective: To investigate the effects of Saluang Belum root infusion on sperm cell survival and testicle histology in mice (Mus musculus). Method: L. sarmentosa root was extracted using an infusion method, analysed and administered to mice at doses of 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW. Aquadest was used as a negative control for 15 days. On the 16th day, sperm motility and viability were assessed. The right testicles were removed and examined under a microscope at 100x and 400x magnification for five fields of view to examine mice testicle histology.     Result: Infusion of L. sarmentosa contained triterpenoids, flavonoids, steroids, alkaloids, saponins, and phenols. Statistical tests of infused L. sarmentosa root on the motility, viability sperm and spermatogenic potentials of mice indicated that the doses of 200mg/kgBW, 400mg/kgBW, and 600mg/kgBW are significantly increased than control.     Conclusion: L. sarmentosa root infusion increased sperm motility and viability, and the spermatogenic cells, as well as Leydig cells in mice.