Characterizing an amidase and its operon from actinomycete bacteria responsible for paraben catabolism

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-06-17 DOI:10.1093/bbb/zbae083

M. Takenoya, Yoshiaki Hiratsuka, K. Shimamura, Shinsaku Ito, Yasuyuki Sasaki, Shunsuke Yajima

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引用次数: 0

Abstract

Hydrazidase from Microbacterium hydrocarbonoxydans was revealed to catalyze synthetic hydrazide compounds, enabling the bacteria to grow with them as sole carbon source, but natural substrates have remained unknown. In this study, kinetic analyses of hydrazidase with parabens showed that the compounds can be substrates. Then, methylparaben induced gene expressions of the operon containing hydrazidase and ABC transporter, and the compound as sole carbon source was able to grow the bacteria. Furthermore, homology search was carried out revealing that several actinomycetes possess hydrazidase-homolog in the operon. Among those bacteria, an amidase from Pseudonocardia acaciae was subjected to a kinetic analysis and a structure determination revealing similar but not identical to those of hydrazidase. Since parabens are reported to exist in plants and soil, and several actinomycetes codes the homologous operon, the enzymes with those operons may play a physiologically important role for bacterial survival with use of parabens.

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放线菌中一种负责对羟基苯甲酸酯分解的酰胺酶及其操作子的特征

研究发现，烃氧微囊藻中的肼酶可以催化合成的肼类化合物，使细菌能够以这些化合物为唯一碳源生长，但天然底物仍是未知数。在本研究中，肼酶与对羟基苯甲酸酯的动力学分析表明，对羟基苯甲酸酯可以作为底物。然后，对羟基苯甲酸甲酯诱导了含有肼酶和 ABC 转运体的操作子的基因表达，并且该化合物作为唯一碳源能够使细菌生长。此外，通过同源检索发现，有几种放线菌的操作子中含有肼酶同源物。在这些细菌中，对来自金合欢假丝酵母的一种酰胺酶进行了动力学分析和结构测定，发现其与肼酶的结构相似，但并不完全相同。据报道，植物和土壤中存在对羟基苯甲酸酯，而几种放线菌编码同源操作子，因此这些操作子中的酶可能对细菌利用对羟基苯甲酸酯生存起着重要的生理作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).