A Rat Model of Pouchitis Following Proctocolectomy and Ileal Pouch-Anal Anastomosis Using Dextran Sulfate Sodium.

IF 1.2 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Jove-Journal of Visualized Experiments Pub Date : 2024-05-31 DOI:10.3791/66623

Anqi He, Song Wang, Kaiyu Li, Baosong Li, Wanyi Xiao, Gang Liu

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Abstract

Ulcerative colitis (UC) is a chronic immune-mediated disease that affects the entire colon and rectum with a relapsing and remitting course, causing lifelong morbidity. When medical treatment is ineffective, especially in cases of massive gastrointestinal bleeding, perforation, toxic megacolon, or carcinogenesis, surgery becomes the last line of defense to cure UC. Total colorectal resection and ileal pouch-anal anastomosis (IPAA) offer the best chance for long-term treatment. Pouchitis is the most common and troublesome postoperative complication. In this investigation, microsurgery is employed to create an ileal pouch model in experimental rats via IPAA surgery. Subsequently, a sustained rat model of pouchitis is established by inducing inflammation of the ileal pouch with dextran sulfate sodium (DSS). The successful establishment of rat pouchitis is validated through analysis of postoperative general status, weight, food and water intake, fecal data, as well as pouch tissue pathology, immunohistochemistry, and inflammatory factor analysis. This experimental animal model of pouchitis provides a foundation for studying the pathogenesis and treatment of the condition.

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使用葡聚糖硫酸钠进行直肠切除术和回肠袋-肛门吻合术后的大鼠袋炎模型

溃疡性结肠炎（UC）是一种由免疫介导的慢性疾病，影响整个结肠和直肠，病程呈复发和缓解状态，导致终生发病。当药物治疗无效时，尤其是在出现大量消化道出血、穿孔、中毒性巨结肠或癌变的情况下，手术就成了治愈 UC 的最后一道防线。全结直肠切除术和回肠袋-肛门吻合术（IPAA）为长期治疗提供了最佳机会。肠袋炎是最常见、最麻烦的术后并发症。本研究采用显微外科技术，通过 IPAA 手术在实验鼠体内建立回肠袋模型。随后，通过使用葡聚糖硫酸钠（DSS）诱导回肠袋发炎，建立了持续的大鼠回肠袋炎模型。通过分析大鼠术后的一般状况、体重、食物和水摄入量、粪便数据以及肠袋组织病理学、免疫组化和炎症因子分析，验证了大鼠肠袋炎模型的成功建立。这种胃袋炎实验动物模型为研究胃袋炎的发病机制和治疗方法奠定了基础。

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来源期刊

Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-

CiteScore

2.10

自引率

0.00%

发文量

992

期刊介绍： JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.

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