High-Resolution Tissue Doppler and Strain Imaging for Adult Zebrafish Myocardial Tissue Through Ultrafast High-Frequency Ultrasound Vector Doppler Estimation

IF 3 2区 工程技术 Q1 ACOUSTICS
Hsin Huang;Alexander Machikhin;De-Quan Chen;Chih-Chung Huang
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Abstract

Zebrafish has been considered as an essential small-animal model for investigating the mechanism of heart regeneration. Due to the small size of zebrafish heart, high-frequency ultrasound (HFUS) imaging is often required for in vivo evaluations of its dynamic functions. Although commercial HFUS systems are available for myocardial velocity and strain measurement, only the outer myocardial region can be quantified due to the complex structure of zebrafish heart. In this study, a high-resolution 2-D myocardial tissue Doppler and strain imaging based on ultrafast HFUS imaging was developed for zebrafish heart imaging during heart regeneration. The cardiac flow region was first extracted to recognize the myocardial region, and the myocardial velocity and strain were then determined through vector Doppler estimation. Adult AB-line zebrafish was used for in vivo experiments, and cryoinjury was induced in the apical region of the heart. Both the myocardial velocity and strain of the whole ventricle after cryoinjury were directly visualized over 28 days. Myocardial velocity (during later diastolic motion) and strain, respectively, were significantly decreased (anterior wall: −2.0 mm/s and −3.3%; apical region: −2.0 mm/s and −4.5%; and posterior wall (PW): −1.7 mm/s and −4.3%) at the first three days after cryoinjury, which indicates weak myocardial beating due to heart injury. However, these all returned to the baseline values at 14 days after cryoinjury. All of the experimental results indicate that the proposed method is a useful tool for heart regeneration studies in adult zebrafish. In particular, it allows for the noninvasive evaluation of regional dynamic heart function.
通过超快高频超声矢量多普勒估算对成年斑马鱼心肌组织进行高分辨率组织多普勒和应变成像。
斑马鱼一直被认为是研究心脏再生机制的重要小动物模型。由于斑马鱼心脏体积小,通常需要使用高频超声(HFUS)成像技术对其动态功能进行体内评估。虽然商用高频超声系统可用于心肌速度和应变测量,但由于斑马鱼心脏结构复杂,只能对心肌外层区域进行量化。本研究开发了一种基于超快 HFUS 成像的高分辨率二维心肌组织多普勒和应变成像技术,用于斑马鱼心脏再生过程中的成像。首先提取心流区域以识别心肌区域,然后通过矢量多普勒估算确定心肌速度和应变。利用成年 AB 线斑马鱼进行体内实验,并在心脏顶端区域诱导冷冻损伤。直接观察冷冻损伤后 28 天内整个心室的心肌速度和应变。心肌速度(舒张后期运动时)和应变分别显著下降(前壁-2.0 mm/s,后壁-2.0 mm/s):前壁:-2.0 毫米/秒和-3.3%;心尖区:-2.0 毫米/秒和-4.5%;后壁:-1.7 毫米/秒和-4.5%):冷冻损伤后最初 3 天,心肌搏动明显减弱(前壁:-2.0 mm/s,-3.3%;心尖区:-2.0 mm/s,-4.5%;后壁:-1.7 mm/s,-4.3%)。然而,在冷冻损伤后 14 天,这些值都恢复到基线值。所有实验结果都表明,所提出的方法是研究成年斑马鱼心脏再生的有用工具。特别是,它可以对区域动态心脏功能进行无创评估。
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来源期刊
CiteScore
7.70
自引率
16.70%
发文量
583
审稿时长
4.5 months
期刊介绍: IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control includes the theory, technology, materials, and applications relating to: (1) the generation, transmission, and detection of ultrasonic waves and related phenomena; (2) medical ultrasound, including hyperthermia, bioeffects, tissue characterization and imaging; (3) ferroelectric, piezoelectric, and piezomagnetic materials, including crystals, polycrystalline solids, films, polymers, and composites; (4) frequency control, timing and time distribution, including crystal oscillators and other means of classical frequency control, and atomic, molecular and laser frequency control standards. Areas of interest range from fundamental studies to the design and/or applications of devices and systems.
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