ScRNA-seq revealed the tumor microenvironment heterogeneity related to the occurrence and metastasis in upper urinary tract urothelial carcinoma

IF 4.8 3区医学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cancer gene therapy Pub Date : 2024-06-14 DOI:10.1038/s41417-024-00779-3

Shiyong Xin, Yanwei Zhang, Zhenhua Zhang, Ziyao Li, Xianchao Sun, Xiang Liu, Liang Jin, Weiyi Li, Chaozhi Tang, Wangli Mei, Qiong Cao, Haojie Wang, Zhihao Wei, Zhen Zhou, Rongbing Li, Xiaofei Wen, Guosheng Yang, Weihua Chen, Junhua Zheng, Lin Ye

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Abstract

Metastasis is the greatest clinical challenge for UTUCs, which may have distinct molecular and cellular characteristics from earlier cancers. Herein, we provide single-cell transcriptome profiles of UTUC para cancer normal tissue, primary tumor lesions, and lymphatic metastases to explore possible mechanisms associated with UTUC occurrence and metastasis. From 28,315 cells obtained from normal and tumor tissues of 3 high-grade UTUC patients, we revealed the origin of UTUC tumor cells and the homology between metastatic and primary tumor cells. Unlike the immunomicroenvironment suppression of other tumors, we found no immunosuppression in the tumor microenvironment of UTUC. Moreover, it is imperative to note that stromal cells are pivotal in the advancement of UTUC. This comprehensive single-cell exploration enhances our comprehension of the molecular and cellular dynamics of metastatic UTUCs and discloses promising diagnostic and therapeutic targets in cancer-microenvironment interactions.

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ScRNA-seq揭示了与上尿路尿路上皮癌发生和转移相关的肿瘤微环境异质性。

转移是UTUC面临的最大临床挑战，UTUC可能具有不同于早期癌症的分子和细胞特征。在此，我们提供了UTUC副癌正常组织、原发肿瘤病灶和淋巴转移的单细胞转录组图谱，以探讨与UTUC发生和转移相关的可能机制。我们从3名高级别UTUC患者的正常组织和肿瘤组织中获得了28 315个细胞，揭示了UTUC肿瘤细胞的来源以及转移细胞和原发肿瘤细胞之间的同源性。与其他肿瘤的免疫微环境抑制不同，我们在UTUC的肿瘤微环境中没有发现免疫抑制。此外，必须指出的是，基质细胞在UTUC的发展中起着关键作用。这种全面的单细胞探索增强了我们对转移性UTUC的分子和细胞动态的理解，并揭示了癌症与微环境相互作用中具有前景的诊断和治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer gene therapy 医学-生物工程与应用微生物

CiteScore

10.20

自引率

0.00%

发文量

150

审稿时长

4-8 weeks

期刊介绍： Cancer Gene Therapy is the essential gene and cellular therapy resource for cancer researchers and clinicians, keeping readers up to date with the latest developments in gene and cellular therapies for cancer. The journal publishes original laboratory and clinical research papers, case reports and review articles. Publication topics include RNAi approaches, drug resistance, hematopoietic progenitor cell gene transfer, cancer stem cells, cellular therapies, homologous recombination, ribozyme technology, antisense technology, tumor immunotherapy and tumor suppressors, translational research, cancer therapy, gene delivery systems (viral and non-viral), anti-gene therapy (antisense, siRNA & ribozymes), apoptosis; mechanisms and therapies, vaccine development, immunology and immunotherapy, DNA synthesis and repair. Cancer Gene Therapy publishes the results of laboratory investigations, preclinical studies, and clinical trials in the field of gene transfer/gene therapy and cellular therapies as applied to cancer research. Types of articles published include original research articles; case reports; brief communications; review articles in the main fields of drug resistance/sensitivity, gene therapy, cellular therapy, tumor suppressor and anti-oncogene therapy, cytokine/tumor immunotherapy, etc.; industry perspectives; and letters to the editor.