Expression and Clinical Significance of Ki-67, CD10, BCL6, MUM1, c-MYC, and EBV in Diffuse Large B Cell Lymphoma Patients.

IF 1.3 4区 医学 Q3 ANATOMY & MORPHOLOGY
Alireza Sadeghipour, Seyed Reza Taha, Mahdieh Shariat Zadeh, Farid Kosari, Pegah Babaheidarian, Fahimeh Fattahi, Navid Abdi, Fatemeh Tajik
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引用次数: 0

Abstract

Introduction: Diffuse large B cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma (NHL) in adults. Although studies regarding the association between the expression of Ki-67, CD10, BCL6, and MUM1 proteins, as well as c-MYC amplification and EBV status with clinicopathologic characteristics have rapidly progressed, their co-expression and prognostic role remain unsatisfactory. Therefore, this study aimed to investigate the association between the expression of all markers and clinicopathologic features and their prognostic value in DLBCL. Also, the co-expression of markers was investigated.

Methods: The protein expression levels and prognostic significance of Ki-67, CD10, BCL6, and MUM1 were investigated with clinical follow-up in a total of 53 DLBCL specimens (including germinal center B [GCB] and activated B cell [ABC] subtypes) as well as adjacent normal samples using immunohistochemistry (IHC). Besides, the clinical significance and prognostic value of c-MYC and EBV status were also evaluated through chromogenic in situ hybridization (CISH), and their correlation with other markers was also assessed.

Results: The results demonstrated a positive correlation between CD10 and BCL6 expression, with both markers being associated with the GCB subtype ( P< 0.001 and P =0.001, respectively). Besides, we observe a statistically significant association between MUM1 protein expression and clinicopathologic type ( P< 0.005) as well as a positive association between c-MYC and recurrence ( P =0.028). Our survival analysis showed that patients who had responded to R-CHOP treatment had better overall survival (OS) and progression-free survival (PFS) than those who did not.

Conclusion: Collectively, this study's results add these markers' value to the existing clinical understanding of DLBCL. However, further investigations are needed to explore markers' prognostic and biological roles in DLBCL patients.

弥漫大 B 细胞淋巴瘤患者中 ki-67、CD10、BCL6、MUM1、c-MYC 和 EBV 的表达及临床意义
导言:弥漫大B细胞淋巴瘤(DLBCL)是成人非霍奇金淋巴瘤(NHL)中最常见的类型。尽管有关 Ki-67、CD10、BCL6 和 MUM1 蛋白表达以及 c-MYC 扩增和 EBV 状态与临床病理特征之间关系的研究进展迅速,但它们的共同表达和预后作用仍不令人满意。因此,本研究旨在探讨 DLBCL 中所有标志物的表达与临床病理特征之间的关联及其预后价值。此外,还对标记物的共表达进行了研究:方法:采用免疫组化(IHC)方法,对53例DLBCL标本(包括生殖中心B细胞[GCB]和活化B细胞[ABC]亚型)及邻近正常标本进行临床随访,研究了Ki-67、CD10、BCL6和MUM1的蛋白表达水平和预后意义。此外,还通过色原原位杂交(CISH)评估了 c-MYC 和 EBV 状态的临床意义和预后价值,并评估了它们与其他标记物的相关性:结果:研究结果表明,CD10和BCL6的表达呈正相关,这两个标志物均与GCB亚型(PC)相关:总之,这项研究的结果为现有的DLBCL临床认识增添了这些标记物的价值。然而,还需要进一步的研究来探讨标记物在DLBCL患者中的预后和生物学作用。
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来源期刊
Applied Immunohistochemistry & Molecular Morphology
Applied Immunohistochemistry & Molecular Morphology ANATOMY & MORPHOLOGY-MEDICAL LABORATORY TECHNOLOGY
CiteScore
3.20
自引率
0.00%
发文量
153
期刊介绍: ​Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist. Official Journal of the International Society for Immunohistochemisty and Molecular Morphology​.
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