Biological control of a novel strain Bacillus velezensis CMML21–47 against sweet potato wilt and black rot diseases

IF 3.7 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control Pub Date : 2024-05-29 DOI:10.1016/j.biocontrol.2024.105541

Yoeng-Seok Yoon , Ju Gyeong Lee , Narayan Chandra Paul , Soyoon Park , Sojung Ryu , Hyunsook Kim , Hyeong Woo Kim , Seonghun Im , Hyunkyu Sang , Gui Hwan Han

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引用次数: 0

Abstract

A root bacterium identified as Bacillus velezensis CMML21–47 with strong biocontrol activity against two major sweet potato fungal diseases caused by Fusarium oxysporum and Ceratocystis fimbriata was isolated in this study. In vitro bioassays and field assays were conducted to test the biocontrol activity and confirmed its strong antagonistic activity. The complete genome of B. velezensis CMML21–47 was sequenced, and multiple gene clusters related to the synthesis of antibiotics by antiSMASH analysis were detected. Selective ultra–high performance liquid chromatography with quadrupole time–of–flight mass spectrometry (UPLC–Q–TOF–MS) was performed and detected lipopeptides, including fengycin, surfactin, and bacillomycin. Lipopeptide fractions were separated, tested against the pathogens, and showed strong growth inhibition. Accordingly, CMML21-47 is an attractive antifungal agent, with high potential to control the sweet potato pathogens causing wilt and black rot diseases.

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新型菌株枯草芽孢杆菌 CMML21-47 防治甘薯枯萎病和黑腐病的生物防治技术

本研究分离了一种根部细菌，经鉴定为枯草芽孢杆菌 CMML21-47，该细菌对由 Fusarium oxysporum 和 Ceratocystis fimbriata 引起的两种主要甘薯真菌病害具有很强的生物防治活性。研究人员进行了体外生物测定和田间试验来检验其生物防治活性，结果证实其具有很强的拮抗活性。对 B. velezensis CMML21-47 的完整基因组进行了测序，并通过 antiSMASH 分析检测到多个与合成抗生素相关的基因簇。采用选择性超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）检测了脂肽，包括芬吉星、表面活性素和杆菌霉素。分离出的脂肽馏分对病原体进行了测试，结果表明具有很强的生长抑制作用。因此，CMML21-47 是一种有吸引力的抗真菌剂，在控制导致枯萎病和黑腐病的甘薯病原体方面具有很大的潜力。

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来源期刊

Biological Control 生物-昆虫学

CiteScore

7.40

自引率

7.10%

发文量

220

审稿时长

63 days

期刊介绍： Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.