Screening of potential α-glucosidase inhibitors from astragalus membranaceus by affinity ultrafiltration screening coupled with UPLC-ESI-Orbitrap-MS method

IF 1.3 3区医学 Q3 CHEMISTRY, APPLIED

Journal of Asian Natural Products Research Pub Date : 2024-06-12 DOI:10.1080/10286020.2024.2366007

引用次数: 0

Abstract

Astragalus membranaceus is a traditional Chinese medicine with multiple pharmacological activities. Modern pharmacological research has found that Astragalus membranaceus extract has an inhibitory effect on α-glucosidase, however, which component can inhibit the activity of α-glucosidase and its degree of inhibition are unknown. To address this issue, this study used affinity ultrafiltration screening combined with UPLC-ESI-Orbitrap-MS technology to screen α-glucosidase inhibitors in Astragalus membranaceus. Using affinity ultrafiltration technology, we obtained the active components, and using UPLC-ESI-Orbitrap-MS technology, we quickly analyzed and identified them. As a result, a total of 8 ingredients were selected as α-glucosidase inhibitors.

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用 UPLC-ESI-Orbitrap-MS 法结合亲和超滤筛选黄芪中潜在的 α-葡萄糖苷酶抑制剂。

黄芪是一种具有多种药理活性的传统中药。现代药理研究发现，黄芪提取物对α-葡萄糖苷酶有抑制作用，但究竟哪种成分能抑制α-葡萄糖苷酶的活性及其抑制程度尚不清楚。针对这一问题，本研究采用亲和超滤筛选结合 UPLC-ESI-Orbitrap-MS 技术筛选了黄芪中的α-葡萄糖苷酶抑制剂。我们利用亲和超滤技术获得了黄芪中的活性成分，并利用 UPLC-ESI-Orbitrap-MS 技术对其进行了快速分析和鉴定。结果，共筛选出 8 种成分作为α-葡萄糖苷酶抑制剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Asian Natural Products Research 医学-药学

CiteScore

3.20

自引率

5.90%

发文量

审稿时长

2.3 months

期刊介绍： The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures. All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.