Comparative functional characterization and in vitro immunological cross-reactivity studies on Daboia russelii and Craspedocephalus malabaricus venom.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-01 DOI:10.1093/trstmh/trae038

Karthika Rajan, Aswathy Alangode, Jaideep C Menon, Dileepkumar Raveendran, Sudarslal Sadasivan Nair, Margaret Reick, Bipin Gopalakrishnan Nair, Martin Reick, Muralidharan Vanuopadath

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引用次数: 0

Abstract

Background: Snake venom is a complex mixture of organic and inorganic constituents, including proteins and peptides. Several studies showed that antivenom efficacy differs due to intra- and inter-species venom variation.

Methods: In the current study, comparative functional characterization of major enzymatic proteins present in Craspedocephalus malabaricus and Daboia russelii venom was investigated through various in vitro and immunological cross-reactivity assays.

Results: The enzymatic assays revealed that hyaluronidase and phospholipase A2 activities were markedly higher in D. russelii. By contrast, fibrinogenolytic, fibrin clotting and L-amino acid oxidase activities were higher in C. malabaricus venom. ELISA results suggested that all the antivenoms had lower binding potential towards C. malabaricus venom. For D. russelii venom, the endpoint titration value was observed at 1:72 900 for all the antivenoms. In the case of C. malabaricus venom, the endpoint titration value was 1:2700, except for Biological E (1:8100). All these results, along with the avidity assays, indicate the strength of venom-antivenom interactions. Similarly, the western blot results suggest that all the antivenoms showed varied efficacies in binding and detecting the venom antigenic epitopes in both species.

Conclusions: The results highlight the need for species-specific antivenom to better manage snakebite victims.

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Daboia russelii 和 Craspedocephalus malabaricus 毒液的功能特性比较和体外免疫交叉反应研究。

背景：蛇毒是一种复杂的有机和无机混合物，其中包括蛋白质和肽。一些研究表明，由于种内和种间毒液的差异，抗蛇毒血清的效力也不同：在本研究中，通过各种体外和免疫学交叉反应试验，研究了马拉巴里鼬和鲁塞尔鼬毒液中主要酶蛋白的功能特性：结果：酶测定结果显示，D. russelii 的透明质酸酶和磷脂酶 A2 活性明显较高。相比之下，马拉巴里茨毒液中的纤维蛋白原溶解活性、纤维蛋白凝固活性和 L-氨基酸氧化酶活性较高。酶联免疫吸附试验结果表明，所有抗蛇毒血清与马拉巴里茨蛇毒的结合潜力都较低。对于 D. russelii 毒液，所有抗蛇毒血清的终点滴定值均为 1:72 900。对于马拉巴里茨毒液，除生物 E（1:8100）外，终点滴定值均为 1:2700。所有这些结果，连同亲和力测定，都表明了毒液与抗蛇毒血清相互作用的强度。同样，Western 印迹结果表明，所有抗蛇毒血清在结合和检测两个物种的蛇毒抗原表位方面都表现出不同的功效：结论：研究结果突出表明，需要针对不同物种的抗蛇毒血清来更好地治疗蛇咬伤患者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.