An examination of the effect of exercise and creatine monohydrate on oral tissues

IF 2 3区工程技术 Q2 ANATOMY & MORPHOLOGY

Microscopy Research and Technique Pub Date : 2024-06-11 DOI:10.1002/jemt.24626

Mehmet Sinan Dogan, Hakim Celik, Sibel Türedi, Abdullah Taskın, Mehmet Emin Dogan, Şemsettin Yıldız

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引用次数: 0

Abstract

Although physical exercise is extremely important for health and a good lifestyle, it can trigger oxidative stress, inflammation, and muscle fatigue. The aim of this study was to determine changes in dental tissues and the mandible created by creatines monohydrate (CrM) supplementation together with low and high-intensity exercise (HIE). The study material comprised Balb/c male mices, which were separated into two groups for the application of low and HIE on a running band. CrM supplement was administered together with the exercise. At the end of the experiment period, dental tissue samples were surgically removed and examined histopathologically and immunohistochemically (TNF-α and lL-1β).As a result of the histopathological examinations, in the pulp, oedema, vascular congestion, and capillary dilatation were seen to be statistically significantly increased in the Group 3 mices that performed HIE compared to the control group (p = 0.001, p = 0.003, p = 0.001, respectively). A statistically significant increase was observed in periodontal ligament (PDL) degeneration, and disruption of the continuity and separation of collagen fibers in Group 3 compared to the control group (p = 0.001). In the immunohistochemical examination, TNF-α and IL-1β positivity was observed in Group 3, and this was significantly increased compared to the control group (p = 0.001, p = 0.000).Exposure of the mices to low and HIE caused histological and immunohistochemical changes in dental pulp and PDL, and it was determined that the use of CrM could have a protective effect against these changes.

Research Highlights

The results of this study showed negative effects of HIE in the dental pulp and PDL, which play an important role in dental health.
CrM was seen to be effective in preventing these negative effects.

Abstract Image

查看原文本刊更多论文

研究运动和一水肌酸对口腔组织的影响。

虽然体育锻炼对健康和良好的生活方式极为重要，但它也会引发氧化应激、炎症和肌肉疲劳。本研究旨在确定在补充一水肌酸（CrM）的同时进行低强度和高强度运动（HIE）对牙齿组织和下颌骨造成的变化。研究材料包括 Balb/c 雄性小鼠，将其分为两组，分别在跑步带上进行低强度和高强度运动。小鼠在运动的同时补充 CrM。组织病理学检查结果显示，与对照组相比，进行 HIE 的第 3 组小鼠的牙髓水肿、血管充血和毛细血管扩张在统计学上显著增加（分别为 p = 0.001、p = 0.003 和 p = 0.001）。与对照组相比，第 3 组小鼠的牙周韧带（PDL）变性、胶原纤维的连续性和分离性遭到破坏的情况在统计学上有明显增加（p = 0.001）。在免疫组化检查中，第 3 组观察到 TNF-α 和 IL-1β 阳性，与对照组相比显著增加（p = 0.001，p = 0.000）。将小鼠暴露于低浓度和 HIE 会导致牙髓和牙周韧带发生组织学和免疫组化变化，而使用 CrM 可以对这些变化起到保护作用。研究亮点：研究结果表明，HIE 对牙髓和 PDL 有负面影响，而牙髓和 PDL 对牙齿健康起着重要作用。CrM 可以有效预防这些负面影响。

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来源期刊

Microscopy Research and Technique 医学-解剖学与形态学

CiteScore

5.30

自引率

20.00%

发文量

233

审稿时长

4.7 months

期刊介绍： Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.