RBD mutations at the residues K417, E484, N501 reduced immunoreactivity with antisera from vaccinated and COVID-19 recovered patients.

IF 2 Q3 PHARMACOLOGY & PHARMACY
Drug Target Insights Pub Date : 2024-05-31 eCollection Date: 2024-01-01 DOI:10.33393/dti.2024.3059
Dablu Lal Gupta, Jhasketan Meher, Anjan Kumar Giri, Arvind K Shukla, Eli Mohapatra, Manisha M Ruikar, D N Rao
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引用次数: 0

Abstract

Introduction: It is unclear whether induced spike protein-specific antibodies due to infections with SARS-CoV-2 or to the prototypic Wuhan isolate-based vaccination can immune-react with the emerging variants of SARS-CoV-2.

Aim/objectives: The main objective of the study was to measure the immunoreactivity of induced antibodies postvaccination with Covishield™ (ChAdOx1 nCoV-19 coronavirus vaccines) or infections with SARS-CoV-2 by using selected peptides of the spike protein of wild type and variants of SARS-CoV-2.

Methodology: Thirty patients who had recovered from SARS-CoV-2 infections and 30 individuals vaccinated with both doses of Covishield™ were recruited for the study. Venous blood samples (5 mL) were collected at a single time point from patients within 3-4 weeks of recovery from SARS-CoV-2 infections or receiving both doses of Covishield™ vaccines. The serum levels of total immunoglobulin were measured in both study groups. A total of 12 peptides of 10 to 24 amino acids length spanning to the receptor-binding domain (RBD) of wild type of SARS-CoV-2 and their variants were synthesized. The serum levels of immune-reactive antibodies were measured using these peptides.

Results: The serum levels of total antibodies were found to be significantly (p<0.001) higher in the vaccinated individuals as compared to COVID-19 recovered patients. Our study reported that the mutations in the RBD at the residues K417, E484, and N501 have been associated with reduced immunoreactivity with anti-sera of vaccinated people and COVID-19 recovered patients.

Conclusion: The amino acid substitutions at the RBD of SARS-CoV-2 have been associated with a higher potential to escape the humoral immune response.

K417、E484和N501残基的RBD突变降低了疫苗接种者和COVID-19康复者抗血清的免疫反应性。
导言:目前还不清楚因感染 SARS-CoV-2 或接种基于武汉分离株的原型疫苗而诱导的尖峰蛋白特异性抗体能否与新出现的 SARS-CoV-2 变体发生免疫反应:该研究的主要目的是通过使用野生型和变异型SARS-CoV-2尖峰蛋白的特定肽,测量接种Covishield™(ChAdOx1 nCoV-19冠状病毒疫苗)或感染SARS-CoV-2后诱导抗体的免疫反应性:研究招募了 30 名 SARS-CoV-2 感染康复患者和 30 名接种过两种剂量 Covishield™ 疫苗的患者。在单个时间点采集 SARS-CoV-2 感染康复后 3-4 周内或接种过两剂 Covishield™ 疫苗的患者的静脉血样本(5 mL)。两组研究人员的血清总免疫球蛋白水平都进行了测定。共合成了 12 个长度为 10 至 24 个氨基酸的肽,这些肽跨越了 SARS-CoV-2 野生型及其变体的受体结合域(RBD)。用这些肽测定了血清中免疫反应性抗体的水平:结果:发现血清中的总抗体水平明显下降(p):结论:SARS-CoV-2 的 RBD 氨基酸置换与较高的逃避体液免疫反应的可能性有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Drug Target Insights
Drug Target Insights PHARMACOLOGY & PHARMACY-
CiteScore
2.70
自引率
0.00%
发文量
5
审稿时长
8 weeks
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