Crystal-packing analysis of translation initiation factor 2 reveals new details of its function.

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Acta Crystallographica. Section D, Structural Biology Pub Date : 2024-07-01 Epub Date: 2024-06-07 DOI:10.1107/S2059798324004029

O S Nikonov, E Y Nikonova, N V Lekontseva, N A Nevskaya, S V Nikonov

引用次数: 0

Abstract

Eukaryotic and archaeal translation initiation factor 2 in complex with GTP delivers the initiator methionyl-tRNA to the small ribosomal subunit. Over the past 20 years, thanks to the efforts of various research groups, including ours, this factor from the archaeon Sulfolobus solfataricus and its individual subunits have been crystallized in ten different space groups. Analysis of the molecular packing in these crystals makes it possible to better understand the roles of functionally significant switches and other elements of the nucleotide-binding pocket during the function of the factor as well as the influence of external effects on its transition between active and inactive states.

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翻译启动因子 2 的晶体堆积分析揭示了其功能的新细节。

真核生物和古生菌翻译起始因子 2 与 GTP 复合物将起始因子蛋氨酰-tRNA 运送到核糖体小亚基。在过去的 20 年中，在包括我们在内的多个研究小组的努力下，这种来自古生菌 Sulfolobus solfataricus 的因子及其各个亚基已经在 10 个不同的空间群中结晶。通过分析这些晶体中的分子排列，可以更好地了解核苷酸结合口袋中具有重要功能的开关和其他元素在该因子的功能过程中所起的作用，以及外部效应对其在活性和非活性状态之间转换的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Crystallographica. Section D, Structural Biology BIOCHEMICAL RESEARCH METHODSBIOCHEMISTRY &-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

4.50

自引率

13.60%

发文量

216

期刊介绍： Acta Crystallographica Section D welcomes the submission of articles covering any aspect of structural biology, with a particular emphasis on the structures of biological macromolecules or the methods used to determine them. Reports on new structures of biological importance may address the smallest macromolecules to the largest complex molecular machines. These structures may have been determined using any structural biology technique including crystallography, NMR, cryoEM and/or other techniques. The key criterion is that such articles must present significant new insights into biological, chemical or medical sciences. The inclusion of complementary data that support the conclusions drawn from the structural studies (such as binding studies, mass spectrometry, enzyme assays, or analysis of mutants or other modified forms of biological macromolecule) is encouraged. Methods articles may include new approaches to any aspect of biological structure determination or structure analysis but will only be accepted where they focus on new methods that are demonstrated to be of general applicability and importance to structural biology. Articles describing particularly difficult problems in structural biology are also welcomed, if the analysis would provide useful insights to others facing similar problems.