Alkalimonas mucilaginosa sp. nov. and Alkalimonas cellulosilytica sp. nov. isolated from alkaline Lonar lake, India

IF 1.8 3区 生物学 Q4 MICROBIOLOGY
Sonia Thite, Devika Godbole, Mahima Debnath, Agrima Bhatt, Amit Yadav, Tushar Lodha, Neetha Joseph, Kiran Kirdat, Dibyajyoti Boruah, Roohani Sharma, Amaraja Joshi
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Abstract

Two alkaliphilic, Gram‐stain-negative bacterial strains (MEB004T and MEB108T) were isolated from water samples collected from Lonar lake, India. The phylogenetic analysis of their 16S rRNA gene sequences showed the highest similarity to A. delamerensis DSM 18314T (98.4%), followed by A. amylolytica DSM 18337T and A. collagenimarina JCM 14267T (97.9%). The genome sizes of strains MEB004T and MEB108T were determined to be 3,858,702 and 4,029,814 bp, respectively, with genomic DNA G + C contents of 51.4 and 51.9%. Average Nucleotide Identity, DNA–DNA Hybridization and Amino Acid Identity values between strains (MEB004T and MEB108T) and A. amylolytica DSM 18337T were (82.3 and 85.5), (25.0 and 29.2) and (86.7 and 90.2%). Both novel strains produced industrially important enzymes, such as amylase, lipase, cellulase, caseinase, and chitinase at pH 10 evidenced by the genomic presence of carbohydrate-active enzymes encoding genes. Genomic analyses further identified pH tolerance genes, affirming their adaptation to alkaline Lonar Lake. Dominant fatty acids were Summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, Summed feature 3, Sum In Feature 2 and C12:0 3OH. The prevalent polar lipids included phosphatidyl ethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol. The major respiratory quinone was ubiquinone-8. Based on the polyphasic data, we propose the classification of strains MEB004T and MEB108T as novel species within the genus Alkalimonas assigning the names Alkalimonas mucilaginosa sp. nov. and Alkalimonas cellulosilytica sp. nov., respectively. The type strains are MEB004T (= MCC 5208T = JCM 35954T = NCIMB 15460T) and MEB108T (= MCC 5330T = JCM 35955T = NCIMB 15461T).

Abstract Image

Abstract Image

从印度碱性洛纳尔湖中分离出的Alkalimonas mucilaginosa sp.nov.和Alkalimonas cellulosilytica sp.nov.。
从印度洛纳尔湖采集的水样中分离出两株嗜碱性、革兰氏染色阴性细菌(MEB004T 和 MEB108T)。它们的 16S rRNA 基因序列的系统进化分析表明,它们与 A. delamerensis DSM 18314T 的相似度最高(98.4%),其次是 A. amylolytica DSM 18337T 和 A. collagenimarina JCM 14267T(97.9%)。经测定,菌株 MEB004T 和 MEB108T 的基因组大小分别为 3,858,702 和 4,029,814 bp,基因组 DNA G + C 含量分别为 51.4% 和 51.9%。菌株(MEB004T和MEB108T)与A. amylolytica DSM 18337T之间的平均核苷酸同一性、DNA-DNA杂交和氨基酸同一性值分别为(82.3和85.5)、(25.0和29.2)和(86.7和90.2%)。两株新菌株在 pH 值为 10 时都能产生工业上重要的酶,如淀粉酶、脂肪酶、纤维素酶、酪蛋白酶和几丁质酶,这一点可以从基因组中存在碳水化合物活性酶编码基因得到证明。基因组分析进一步确定了耐受 pH 值的基因,证实了它们对碱性洛纳尔湖的适应性。主要脂肪酸为总特征 8(C18:1 ω7c 和/或 C18:1 ω6c)、C16:0、总特征 3、总特征 2 和 C12:0 3OH。常见的极性脂质包括磷脂酰乙醇胺、磷脂酰甘油和二磷脂酰甘油。主要的呼吸醌是泛醌-8。根据多相数据,我们建议将 MEB004T 和 MEB108T 菌株归类为 Alkalimonas 属中的新种,分别命名为 Alkalimonas mucilaginosa sp.nov.和 Alkalimonas cellulosilytica sp.nov.。模式菌株为 MEB004T(= MCC 5208T = JCM 35954T = NCIMB 15460T)和 MEB108T(= MCC 5330T = JCM 35955T = NCIMB 15461T)。
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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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