Identifying MiR-140-3p as a stable internal reference to normalize MicroRNA qRT-PCR levels of plasma small extracellular vesicles in lung cancer patients

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Yuan Jiang , Weiwei Wang , Faqing Tang , Wei Zhang , Sheng Chen , Xiumei Gu , Ping Chen , Xiaoya Xu , Baoning Nian , Zhikuan Li , Chunzhu Chen , Hanbing Yin , Linlin Su , Honghou Sun , Wei Chen , Dadong Zhang , Yuejin Li
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Abstract

Exploration of a stably expressed gene as a reference is critical for the accurate evaluation of miRNAs isolated from small extracellular vesicles (sEVs). In this study, we analyzed small RNA sequencing on plasma sEV miRNAs in the training dataset (n = 104) and found that miR-140-3p was the most stably expressed candidate reference for sEV miRNAs. We further demonstrated that miR-140-3p expressed most stably in the validation cohort (n = 46) when compared to two other reference miRNAs, miR-451a and miR-1228-3p, and the commonly-used miRNA reference U6. Finally, we compared the capability of miR-140-3p and U6 as the internal reference for sEV miRNA expression by evaluating key miRNAs expression in lung cancer patients and found that miR-140-3p was more suitable as a sEV miRNA reference gene. Taken together, our data indicated miR-140-3p as a stable internal reference miRNA of plasma sEVs to evaluate miRNA expression profiles in lung cancer patients.

确定 MiR-140-3p 作为稳定的内参照物,以归一化肺癌患者血浆小细胞外囊泡中的 MicroRNA qRT-PCR 水平。
探索一种稳定表达的基因作为参照物对于准确评估从小细胞外囊泡 (sEV) 分离出来的 miRNA 至关重要。在这项研究中,我们分析了训练数据集中血浆 sEV miRNAs 的小 RNA 测序(n = 104),发现 miR-140-3p 是 sEV miRNAs 中表达最稳定的候选参考基因。我们进一步证明,与另外两个参考 miRNA(miR-451a 和 miR-1228-3p)和常用的 miRNA 参考 U6 相比,miR-140-3p 在验证队列(n = 46)中的表达最稳定。最后,我们通过评估肺癌患者体内关键 miRNA 的表达,比较了 miR-140-3p 和 U6 作为 sEV miRNA 表达内参基因的能力,发现 miR-140-3p 更适合作为 sEV miRNA 的参比基因。综上所述,我们的数据表明 miR-140-3p 是评估肺癌患者血浆 sEV 中 miRNA 表达谱的稳定的内参 miRNA。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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