The impact of heterotopic ovarian tissue transplantation with varied volumes on reproductive endocrine function in oophorectomized rats.

IF 0.9 4区医学 Q4 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Pub Date : 2024-12-01 Epub Date: 2024-06-07 DOI:10.1080/01443615.2024.2362416

Nana Tian, Chunhui Liu, Zhihui Cai, Junrong Shi, Pin Li, Shasha Bai, Xiaoxin Du, Gaoyuan Liu, Min Geng, Peishan Weng

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引用次数: 0

Abstract

Background: This study aimed to investigate the effects of different volumes of ovarian tissue transplantation on the reproductive endocrine function of rats after oophorectomy.

Methods: Female rats were selected to establish a castration model and then underwent different volumes of ovarian tissue transplantation. Group I served as the sham operation group. The transplantation group was divided into five subgroups based on the calculated ratio of ovarian weight to body weight in normal female rats, δ = (2.52 ± 0.17) ×10^-4: Group II: transplanted ovarian volume was δ; Group III: 0.75δ; Group IV: 0.5δ; Group V: 0.25δ; Group VI: without ovarian transplantation. The post-transplant oestrous cycle recovery was observed, and blood samples were collected every 2 weeks to measure serum hormone levels. Histological evaluation was performed at the end of the observation period.

Results: Rats in Group V exhibited disrupted oestrous cycles after transplantation, which were significantly longer than those in Group I. Rats in Groups II, III, and IV showed no cyclic changes. At 6 weeks post-transplantation, rats in Group V had lower E2 and AMH levels and higher FSH levels compared to Group I. The uterine wet weight and the number of normal follicles in Group V were significantly lower than those in Group I, but the number of atretic follicles was higher than in Group I.

Conclusion: The larger ovarian tissue transplantation resulted in a faster recovery with a higher survival rate of the uterus and normal follicles, compared to smaller ovarian tissue transplantation.

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不同体积的异位卵巢组织移植对卵巢切除大鼠生殖内分泌功能的影响

背景：本研究旨在探讨不同体积卵巢组织移植对大鼠卵巢切除术后生殖内分泌功能的影响：本研究旨在探讨不同体积卵巢组织移植对卵巢切除术后大鼠生殖内分泌功能的影响：方法：选择雌性大鼠建立阉割模型，然后进行不同体积的卵巢组织移植。I 组为假手术组。移植组根据正常雌性大鼠卵巢重量与体重的计算比值δ=（2.52 ± 0.17）×10-4分为五个亚组：Ⅱ组：移植卵巢体积为δ；Ⅲ组：0.75δ；Ⅳ组：0.5δ；Ⅴ组：0.25δ；Ⅵ组：未进行卵巢移植。观察移植后发情周期的恢复情况，每两周采集一次血样，以测定血清激素水平。观察期结束后进行组织学评估：结果：移植后，第五组大鼠的发情周期紊乱，明显长于第一组大鼠。移植后6周时，与I组相比，V组大鼠的E2和AMH水平较低，FSH水平较高。V组大鼠的子宫湿重和正常卵泡数明显低于I组，但闭锁卵泡数高于I组：结论：与较小的卵巢组织移植相比，较大的卵巢组织移植恢复更快，子宫和正常卵泡的存活率更高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Obstetrics and Gynaecology 医学-妇产科学

CiteScore

2.40

自引率

7.70%

发文量

398

审稿时长

6 months

期刊介绍： Journal of Obstetrics and Gynaecology represents an established forum for the entire field of obstetrics and gynaecology, publishing a broad range of original, peer-reviewed papers, from scientific and clinical research to reviews relevant to practice. It also includes occasional supplements on clinical symposia. The journal is read widely by trainees in our specialty and we acknowledge a major role in education in Obstetrics and Gynaecology. Past and present editors have recognized the difficulties that junior doctors encounter in achieving their first publications and spend time advising authors during their initial attempts at submission. The journal continues to attract a world-wide readership thanks to the emphasis on practical applicability and its excellent record of drawing on an international base of authors.