linc01152 Regulates Cell Viability, Cell Migration and Cell Invasion of Breast Cancer via Regulating miR-320a and MTDH.

IF 2.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Genetics Pub Date : 2024-06-07 DOI:10.1007/s10528-024-10851-0

Zhiwei Zhu, Siting Lin, Lihong Pang

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引用次数: 0

Abstract

Breast cancer is a global disease and a cause of cancer-related deaths in women. Long non-coding RNAs (lncRNAs) perform important functions in biological processes. The aim of this study was to verify the functions and regulatory mechanisms of linc01152 in breast cancer. Relative expression of linc01152 was measured using RT-PCR. siRNAs targeting linc01152 were designed to inhibit its expression. Cell viability, cell invasion, and migration capacities were determined using CCK-8 and Transwell assays. Downstream targets, miRNAs, and mRNAs were predicted and validated using luciferase reporter assay. The expression of linc01152 in breast cancer cells was higher than that in normal breast cells, with BT474 and MDA-MB-468 cell lines presenting the highest expression levels of linc01152. The inhibition of linc01152 expression led to lower cell viability and attenuated cell migration and invasion. The regulatory network of linc01152-miR-320a-MTDH was validated using luciferase reporter assay. The inhibition of miR-320a expression reversed the effect of si-linc01152 on cell viability, migration, and invasion. Taken together, the linc01152-miR-320a-MTDH regulatory network is correlated with the pathogenesis of breast cancer.

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linc01152通过调控miR-320a和MTDH调节乳腺癌的细胞活力、细胞迁移和细胞侵袭

乳腺癌是一种全球性疾病，也是女性因癌症死亡的原因之一。长非编码 RNA（lncRNA）在生物过程中发挥着重要功能。本研究旨在验证linc01152在乳腺癌中的功能和调控机制。研究采用 RT-PCR 技术测定了 linc01152 的相对表达量，并设计了靶向 linc01152 的 siRNA 来抑制其表达。使用 CCK-8 和 Transwell 试验测定细胞活力、细胞侵袭和迁移能力。利用荧光素酶报告实验预测并验证了下游靶标、miRNA 和 mRNA。linc01152在乳腺癌细胞中的表达量高于正常乳腺癌细胞，其中BT474和MDA-MB-468细胞株的linc01152表达量最高。抑制 linc01152 的表达会降低细胞的活力，减少细胞的迁移和侵袭。利用荧光素酶报告实验验证了 linc01152-miR-320a-MTDH 的调控网络。抑制 miR-320a 的表达可逆转 si-linc01152 对细胞活力、迁移和侵袭的影响。综上所述，linc01152-miR-320a-MTDH调控网络与乳腺癌的发病机制相关。

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来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.