Comparative metabolic profiling of the mycelium and fermentation broth of Penicillium restrictum from Peucedanum praeruptorum rhizosphere

IF 3.6 4区 生物学 Q2 ENVIRONMENTAL SCIENCES
Yuanyuan Wang, Ranran Liao, Haoyu Pan, Xuejun Wang, Xiaoting Wan, Bangxing Han, Cheng Song
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Abstract

Microorganisms in the rhizosphere, particularly arbuscular mycorrhiza, have a broad symbiotic relationship with their host plants. One of the major fungi isolated from the rhizosphere of Peucedanum praeruptorum is Penicillium restrictum. The relationship between the metabolites of P. restrictum and the root exudates of P. praeruptorum is being investigated. The accumulation of metabolites in the mycelium and fermentation broth of P. restrictum was analysed over different fermentation periods. Non-targeted metabolomics was used to compare the differences in intracellular and extracellular metabolites over six periods. There were significant differences in the content and types of mycelial metabolites during the incubation. Marmesin, an important intermediate in the biosynthesis of coumarins, was found in the highest amount on the fourth day of incubation. The differential metabolites were screened to obtain 799 intracellular and 468 extracellular differential metabolites. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that the highly enriched extracellular metabolic pathways were alanine, aspartate and glutamate metabolism, glyoxylate and dicarboxylate metabolism, and terpenoid backbone biosynthesis. In addition, the enrichment analysis associated with intracellular and extracellular ATP-binding cassette transporter proteins revealed that some ATP-binding cassette transporters may be involved in the transportation of certain amino acids and carbohydrates. Our results provide some theoretical basis for the regulatory mechanisms between the rhizosphere and the host plant and pave the way for the heterologous production of furanocoumarin.

Abstract Image

对 Peucedanum praeruptorum 根瘤菌中限制青霉的菌丝体和发酵液进行比较代谢分析。
根瘤层中的微生物,特别是节肢菌根,与其寄主植物有着广泛的共生关系。从 Peucedanum praeruptorum 根圈中分离出的主要真菌之一是限制青霉(Penicillium restrictum)。目前正在研究 P. restrictum 的代谢物与 Peucedanum praeruptorum 根渗出物之间的关系。分析了不同发酵期 P. restrictum 菌丝体和发酵液中代谢物的积累情况。非靶向代谢组学用于比较六个时期细胞内和细胞外代谢物的差异。在培养过程中,菌丝代谢物的含量和类型存在明显差异。香豆素是香豆素生物合成过程中的重要中间体,在培养的第四天含量最高。通过对差异代谢物的筛选,获得了 799 种细胞内差异代谢物和 468 种细胞外差异代谢物。京都基因和基因组百科全书》途径富集分析表明,细胞外代谢途径富集程度较高的是丙氨酸、天门冬氨酸和谷氨酸代谢,乙醛酸和二羧酸代谢,以及萜类骨架生物合成。此外,与细胞内和细胞外 ATP 结合盒转运蛋白相关的富集分析表明,一些 ATP 结合盒转运蛋白可能参与了某些氨基酸和碳水化合物的转运。我们的研究结果为根瘤菌与寄主植物之间的调控机制提供了一些理论依据,并为呋喃香豆素的异源生产铺平了道路。
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来源期刊
Environmental Microbiology Reports
Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY
CiteScore
6.00
自引率
3.00%
发文量
91
审稿时长
3.0 months
期刊介绍: The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.
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