Chromatographic and Spectrophotometric Determination of Clindamycin in Pharmaceutical Products

IF 1 Q4 CHEMISTRY, MULTIDISCIPLINARY

Indonesian Journal of Chemistry Pub Date : 2024-06-01 DOI:10.22146/ijc.91599

I. Al-Momani, Lana Mohammad Zaid Al-Kilani

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Abstract

Accurate, precise, and reliable chromatographic and spectrophotometric methods were developed for determining clindamycin (CLD) in pharmaceutical formulations. The spectrophotometric method was adopted for flow injection analysis (FIA). The method is based on the online oxidation of CLD and measuring the absorbance of the resulting product using a flow cell at 605 nm. Experimental conditions, including FIA variables and reaction conditions, were optimized. The chromatographic separation was achieved using a C8 column and an isocratic mobile phase. The composition of the mobile phase selected for the analysis consists of a mixture of phosphate buffer (50%), MeOH (35%), and ACN (15%), adjusted to a pH of 3.47 by phosphoric acid. The eluent was monitored with a UV detector at 205 nm. The linearity range was 10–200 and 50–800 µg/mL for the FIA and HPLC, respectively. The applicability of the FIA and HPLC methods was validated by analyzing CLD in synthetic and commercial pharmaceutical products. No significant interferences were observed from the common excipients usually used in commercial formulations.

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色谱法和分光光度法测定药品中的克林霉素

建立了准确、精确、可靠的色谱法和分光光度法测定药物制剂中的克林霉素（CLD）。采用分光光度法进行流动注射分析（FIA）。该方法基于 CLD 的在线氧化，并使用流动池在 605 纳米波长处测量生成物的吸光度。对包括 FIA 变量和反应条件在内的实验条件进行了优化。色谱分离采用 C8 色谱柱和等度流动相。用于分析的流动相由磷酸盐缓冲液（50%）、MeOH（35%）和 ACN（15%）的混合物组成，用磷酸调节 pH 值至 3.47。洗脱液用紫外检测器在 205 纳米波长处进行监测。FIA 和 HPLC 的线性范围分别为 10-200 微克/毫升和 50-800 微克/毫升。通过分析合成和商业药品中的 CLD，验证了 FIA 和 HPLC 方法的适用性。没有发现商业制剂中常用辅料的明显干扰。

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来源期刊

Indonesian Journal of Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

2.30

自引率

11.10%

发文量

106

审稿时长

15 weeks

期刊介绍： Indonesian Journal of Chemistry is a peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry, including educational chemistry, applied chemistry, and chemical engineering.