Determination of the Chemical Content of Shilajit in Terms of Ten Different Polyphenolic Compounds by UAE Method and HPLC Analysis

Abstract

Shilajit (Mumio) is a complex of natural bioactive compounds that has historically been used as a therapeutic cream for many skin diseases and is suitable in traditional medicine for diseases such as diabetes, Alzheimer's and cancer. In this study, to determine the polyphenolic content of Shilajit, extracts obtained at 45 degrees were analyzed by High Performance Liquid Chromatography-Ultraviolet (HPLC-UV) for the quantitative analysis of ten different polyphenolic compounds. As a result of the experimental study, 28.99±1.23 µg/g routine, 25.47±1.67 µg/g ferulic acid, 41.49±0.41 µg/g resveratrol and 532.19±8.21 µg/g taxifolin was obtained. This study's results will significantly contribute to sectors such as the extraction of bioactive substances, scaling studies, and their use as food supplements and pharmaceutical applications.