{"title":"<i>UPBEAT1</i>-ROS-POD-<i>PAL</i> System under Different Xylogenesis Scenarios in Karelian Birch <i>(Betula pendula</i> Roth var. carelica (Mercl.) Hämet-Ahti).","authors":"Kseniya Mihajlovna Nikerova, Nataliya Alekseevna Galibina, Irina Nikolaevna Sofronova, Yuliya Leonidovna Moshchenskaya, Maksim Anatol'evich Korzhenevskij, Anna Vladimirovna Klimova, Tatiana Vladimirovna Tarelkina","doi":"10.2174/0109298665291781240529044444","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>We studied <i>UPBEAT1 (UPB1)</i> which regulated superoxide radical / hydrogen peroxide ratio together with peroxidase (POD) activity and <i>PAL</i> genes expression under different ways of apical meristem development during the xylem structural elements' formation in unique woody plants <i>B. pendula</i> var. pendula with straight-grained wood and <i>B. pendula</i> var. carelica with figured wood. The differentiation process predominanced in straight-grained wood (<i>B. pendula</i> var. <i>pendula</i>) or proliferation - in the figured wood. The investigation was conducted in the radial row (cambial zone - differentiating xylem - mature xylem) during the active cambial growth period.</p><p><strong>Objective: </strong>The study aimed to study the xylogenesis processes occurring in the 16-year-old straight-grained silver birch (<i>Betula pendula</i> Roth) and Karelian birch (<i>Betula pendula</i> Roth var. carelica (Mercl.) Hämet-Ahti) with figured wood.</p><p><strong>Methods: </strong>Hydrogen peroxide and superoxide radical contents and peroxidase activity were determined spectrophotometrically. Gene expression for <i>PAL</i> family genes and the <i>UPBEAT1</i> gene was assessed using qRT-PCR.</p><p><strong>Results: </strong>Principal component analysis has confirmed trees with straight-grained and figured wood to be different according to UPBEAT1-ROS-POD-<i>PAL</i> system functioning.</p><p><strong>Conclusion: </strong>The higher superoxide radical/hydrogen peroxide ratio in figured Karelian birch, along with <i>UPBEAT1</i> transcription factor and <i>PAL</i> genes upregulation, distinguished it from straight-grained silver birch. This metabolic picture confirmed the shift of Karelian birch xylogenesis towards proliferation processes, accompanied by ROS and phenolic compounds' flow and POD activity.</p>","PeriodicalId":20736,"journal":{"name":"Protein and Peptide Letters","volume":" ","pages":"375-385"},"PeriodicalIF":1.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Protein and Peptide Letters","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2174/0109298665291781240529044444","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: We studied UPBEAT1 (UPB1) which regulated superoxide radical / hydrogen peroxide ratio together with peroxidase (POD) activity and PAL genes expression under different ways of apical meristem development during the xylem structural elements' formation in unique woody plants B. pendula var. pendula with straight-grained wood and B. pendula var. carelica with figured wood. The differentiation process predominanced in straight-grained wood (B. pendula var. pendula) or proliferation - in the figured wood. The investigation was conducted in the radial row (cambial zone - differentiating xylem - mature xylem) during the active cambial growth period.
Objective: The study aimed to study the xylogenesis processes occurring in the 16-year-old straight-grained silver birch (Betula pendula Roth) and Karelian birch (Betula pendula Roth var. carelica (Mercl.) Hämet-Ahti) with figured wood.
Methods: Hydrogen peroxide and superoxide radical contents and peroxidase activity were determined spectrophotometrically. Gene expression for PAL family genes and the UPBEAT1 gene was assessed using qRT-PCR.
Results: Principal component analysis has confirmed trees with straight-grained and figured wood to be different according to UPBEAT1-ROS-POD-PAL system functioning.
Conclusion: The higher superoxide radical/hydrogen peroxide ratio in figured Karelian birch, along with UPBEAT1 transcription factor and PAL genes upregulation, distinguished it from straight-grained silver birch. This metabolic picture confirmed the shift of Karelian birch xylogenesis towards proliferation processes, accompanied by ROS and phenolic compounds' flow and POD activity.
期刊介绍：
Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations.
Protein & Peptide Letters focuses on:
Structure Studies
Advances in Recombinant Expression
Drug Design
Chemical Synthesis
Function
Pharmacology
Enzymology
Conformational Analysis
Immunology
Biotechnology
Protein Engineering
Protein Folding
Sequencing
Molecular Recognition
Purification and Analysis
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