Genomic analysis of a halophilic bacterium Nesterenkonia sp. CL21 with ability to produce a diverse group of lignocellulolytic enzymes.

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hongxuan An, Xin Huey Ching, Wai Jun Cheah, Wei Lun Lim, Kah Yaw Ee, Chun Shiong Chong, Ming Quan Lam
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Abstract

Halophilic bacteria are extremophiles that thrive in saline environment. Their ability to withstand such harsh conditions makes them an ideal choice for industrial applications such as lignocellulosic biomass degradation. In this study, a halophilic bacterium with the ability to produce extracellular cellulases and hemicellulases, designated as Nesterenkonia sp. CL21, was isolated from mangrove sediment in Tanjung Piai National Park, Malaysia. Thus far, studies on lignocellulolytic enzymes concerning bacterial species under this genus are limited. To gain a comprehensive understanding of its lignocellulose-degrading potential, the whole genome was sequenced using the Illumina NovaSeq 6000 platform. The genome of strain CL21 was assembled into 25 contigs with 3,744,449 bp and a 69.74% GC content and was predicted to contain 3,348 coding genes. Based on taxonomy analysis, strain CL21 shares 73.8 to 82.0% average nucleotide identity with its neighbouring species, below the 95% threshold, indicating its possible status as a distinct species in Nesterenkonia genus. Through in-depth genomic mining, a total of 81 carbohydrate-active enzymes were encoded. Among these, 24 encoded genes were identified to encompass diverse cellulases (GH3), xylanases (GH10, GH11, GH43, GH51, GH127 and CE4), mannanases (GH38 and GH106) and pectinases (PL1, PL9, and PL11). The production of lignocellulolytic enzymes was tested in the presence of several substrates. This study revealed that strain CL21 can produce a diverse array of enzymes which are active at different time points. By combining experimental data with genomic information, the ability of strain CL21 to produce lignocellulolytic enzymes has been elucidated, with potential applications in biorefinery industry.

Abstract Image

对具有生产多种木质纤维素分解酶能力的嗜卤细菌 Nesterenkonia sp.
嗜卤细菌是在盐碱环境中生长的极端嗜盐菌。它们能够承受如此恶劣的条件,是木质纤维素生物质降解等工业应用的理想选择。在这项研究中,从马来西亚丹绒披耶国家公园的红树林沉积物中分离出了一种能够产生胞外纤维素酶和半纤维素酶的嗜盐细菌,命名为 Nesterenkonia sp.CL21。到目前为止,有关该属细菌物种木质纤维素分解酶的研究还很有限。为了全面了解其木质纤维素降解潜力,利用 Illumina NovaSeq 6000 平台对其进行了全基因组测序。菌株 CL21 的基因组被组装成 25 个等位基因,长度为 3,744,449 bp,GC 含量为 69.74%,预计包含 3,348 个编码基因。根据分类学分析,CL21菌株与邻近物种的平均核苷酸同一性为73.8%至82.0%,低于95%的临界值,表明其可能是Nesterenkonia属中的一个独立物种。通过深入的基因组挖掘,共编码了 81 种碳水化合物活性酶。其中,鉴定出 24 个编码基因,包括多种纤维素酶(GH3)、木聚糖酶(GH10、GH11、GH43、GH51、GH127 和 CE4)、甘露聚糖酶(GH38 和 GH106)和果胶酶(PL1、PL9 和 PL11)。在几种底物存在的情况下,对木质纤维素分解酶的产量进行了测试。这项研究表明,菌株 CL21 能产生多种酶,这些酶在不同的时间点具有活性。通过将实验数据与基因组信息相结合,阐明了菌株 CL21 生产木质纤维素分解酶的能力,并有望应用于生物炼制工业。
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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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