Exosome-Mediated Communication in Thyroid Cancer: Implications for Prognosis and Therapeutic Targets.

IF 2.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Genetics Pub Date : 2024-06-05 DOI:10.1007/s10528-024-10833-2

Yiwei Wang, Qiang Li, Xinrui Yang, Hanyu Guo, Tian Ren, Tianchi Zhang, Pantea Ghadakpour, Fu Ren

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Abstract

Thyroid cancer (THCA) is one of the most common malignancies of the endocrine system. Exosomes have significant value in performing molecular treatments, evaluating the diagnosis and determining tumor prognosis. Thus, the identification of exosome-related genes could be valuable for the diagnosis and potential treatment of THCA. In this study, we examined a set of exosome-related differentially expressed genes (DEGs) (BIRC5, POSTN, TGFBR1, DUSP1, BID, and FGFR2) by taking the intersection between the DEGs of the TCGA-THCA and GeneCards datasets. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the exosome-related DEGs indicated that these genes were involved in certain biological functions and pathways. Protein‒protein interaction (PPI), mRNA‒miRNA, and mRNA-TF interaction networks were constructed using the 6 exosome-related DEGs as hub genes. Furthermore, we analyzed the correlation between the 6 exosome-related DEGs and immune infiltration. The Genomics of Drug Sensitivity in Cancer (GDSC), the Cancer Cell Line Encyclopedia (CCLE), and the CellMiner database were used to elucidate the relationship between the exosome-related DEGs and drug sensitivity. In addition, we verified that both POSTN and BID were upregulated in papillary thyroid cancer (PTC) patients and that their expression was correlated with cancer progression. The POSTN and BID protein expression levels were further examined in THCA cell lines. These findings provide insights into exosome-related clinical trials and drug development.

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外泌体介导的甲状腺癌通讯：对预后和治疗目标的影响

甲状腺癌（THCA）是内分泌系统最常见的恶性肿瘤之一。外泌体在进行分子治疗、评估诊断和确定肿瘤预后方面具有重要价值。因此，鉴定与外泌体相关的基因可能对 THCA 的诊断和潜在治疗很有价值。在本研究中，我们通过对TCGA-THCA和GeneCards数据集中的差异表达基因（DEGs）（BIRC5、POSTN、TGFBR1、DUSP1、BID和FGFR2）进行交叉研究，发现了一组与外泌体相关的差异表达基因（DEGs）。对外泌体相关 DEGs 的基因本体（GO）和京都基因组百科全书（KEGG）分析表明，这些基因参与了某些生物学功能和途径。以这 6 个外泌体相关 DEGs 为中枢基因，构建了蛋白质-蛋白质相互作用（PPI）、mRNA-miRNA 和 mRNA-TF 相互作用网络。此外，我们还分析了 6 个外泌体相关 DEGs 与免疫浸润之间的相关性。我们利用癌症药物敏感性基因组学（GDSC）、癌症细胞系百科全书（CCLE）和CellMiner数据库来阐明外泌体相关DEGs与药物敏感性之间的关系。此外，我们还验证了POSTN和BID在甲状腺乳头状癌（PTC）患者中的上调，并且它们的表达与癌症进展相关。我们还进一步检测了POSTN和BID在THCA细胞系中的蛋白表达水平。这些发现为与外泌体相关的临床试验和药物开发提供了启示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.