Microorganism-mediated denitrogenation of aquaculture systems provoked by poly-β-hydroxybutyrate (PHB)

IF 10.4 1区 工程技术 Q1 ENGINEERING, CHEMICAL
Yun Xia, Yarong Liu, Kai Zhang, Jinhui Sun, Guangjun Wang, Zhifei Li, Jingjing Tian, Wangbao Gong, Hongyan Li, Wenping Xie, Gen Kaneko, Jun Xie, Ermeng Yu
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Abstract

The biodegradable polymer poly-β-hydroxybutyrate (PHB) is a promising carbon source for biological mitigation of nitrogen pollution, a significant problem in aquaculture that physical and chemical methods have not provided a comprehensive solution. Here we investigated the impact of PHB on the zero-water-change largemouth bass culture by 30- and 40-day experiments. PHB loaded into the filter circulation pump at 4 g L−1, optimum value determined by the first experiment, significantly reduced the levels of nitrate by 99.65%, nitrite by 95.96%, and total nitrogen by 85.22% compared to the control without PHB. PHB also significantly increased denitrifying bacteria (e.g., Proteobacteria and Fusobacteria) and expression of denitrification genes (e.g., nirK and nirS) in the microbial community, improving growth and health parameters of largemouth bass. While the impact may vary in other culture systems, PHB thus demonstrated its remarkable utility in aquaculture, highlighting ecological assessment and application to larger aquaculture operations as future considerations.

Abstract Image

Abstract Image

聚-β-羟基丁酸盐(PHB)引发的微生物介导的水产养殖系统脱氮作用
可生物降解的聚合物聚-β-羟基丁酸盐(PHB)是一种很有前景的生物缓解氮污染的碳源,而氮污染是水产养殖中的一个重要问题,物理和化学方法尚未提供全面的解决方案。在此,我们通过 30 天和 40 天的实验研究了 PHB 对零换水大口鲈养殖的影响。与不添加 PHB 的对照组相比,在过滤循环泵中添加 4 g L-1 的 PHB(第一次实验确定的最佳值)可显著降低硝酸盐含量 99.65%、亚硝酸盐含量 95.96%和总氮含量 85.22%。PHB 还能明显增加微生物群落中的反硝化细菌(如变形菌和镰刀菌)和反硝化基因(如 nirK 和 nirS)的表达,从而改善大口鲈鱼的生长和健康参数。虽然 PHB 对其他养殖系统的影响可能不同,但它在水产养殖中的显著效用已得到证明,生态评估和应用于更大规模的水产养殖是未来需要考虑的重点。
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来源期刊
npj Clean Water
npj Clean Water Environmental Science-Water Science and Technology
CiteScore
15.30
自引率
2.60%
发文量
61
审稿时长
5 weeks
期刊介绍: npj Clean Water publishes high-quality papers that report cutting-edge science, technology, applications, policies, and societal issues contributing to a more sustainable supply of clean water. The journal's publications may also support and accelerate the achievement of Sustainable Development Goal 6, which focuses on clean water and sanitation.
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