Ethnopharmacological study on Adenosma buchneroides Bonati inhibiting inflammation via the regulation of TLR4/MyD88/NF-κB signaling pathway

IF 4.8 3区化学 Q1 CHEMISTRY, MEDICINAL

Natural Products and Bioprospecting Pub Date : 2024-06-04 DOI:10.1007/s13659-024-00458-8

Yuru Shi, Xiaoqian Zhang, Shengji Pei, Yuhua Wang

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Abstract

Adenosma buchneroides Bonati, also known as fleagrass, is an important medicinal plant used by the Akha (Hani) people of China for treating inflammation-related skin swelling, acne, and diarrhoea, among other conditions. In this study, we aimed to evaluate the anti-inflammatory activities and explore the molecular mechanisms of fleagrass on treating skin swelling and acne. The results demonstrated that fleagrass inhibited the enzymatic activities of 5-LOX and COX-2 in vitro, and decreased the release of NO, IL-6, TNF-α, and IL-10 in the LPS-induced RAW264.7 macrophages. The levels of proteins associated with the nuclear factor-kappa B (NF-κB) pathway were examined by western blotting and immunofluorescence, demonstrating that fleagrass downregulated the expression of TLR4, MyD88, NF-κB/p65, and iNOS and blocked the nuclear translocation of NF-κB/p65. Furthermore, fleagrass exhibited acute anti-inflammatory activity in paw oedema models. The results confirm that fleagrass exhibits remarkable anti-inflammatory activity and can be used in alleviating inflammation, suggesting that fleagrass has the potential to be a novel anti-inflammatory agent.

Graphical Abstract

Abstract Image

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关于 Adenosma buchneroides Bonati 通过调节 TLR4/MyD88/NF-κB 信号通路抑制炎症的民族药理学研究。

Adenosma buchneroides Bonati，又名飞蓬草，是中国阿卡族（哈尼族）人用来治疗与炎症有关的皮肤肿胀、痤疮和腹泻等疾病的一种重要药用植物。本研究旨在评估飞蓬草的抗炎活性，并探索其治疗皮肤肿胀和痤疮的分子机制。结果表明，飞蓬草在体外能抑制 5-LOX 和 COX-2 的酶活性，减少 LPS 诱导的 RAW264.7 巨噬细胞中 NO、IL-6、TNF-α 和 IL-10 的释放。免疫印迹法和免疫荧光法检测了与核因子-kappa B（NF-κB）通路相关的蛋白质水平，结果表明飞蓬草可降低 TLR4、MyD88、NF-κB/p65 和 iNOS 的表达，并阻断 NF-κB/p65 的核转位。此外，飞蓬草在爪水肿模型中表现出急性抗炎活性。研究结果证实，飞蓬草具有显著的抗炎活性，可用于缓解炎症，表明飞蓬草有潜力成为一种新型抗炎剂。

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来源期刊

Natural Products and Bioprospecting CHEMISTRY, MEDICINAL-

CiteScore

8.30

自引率

2.10%

发文量

审稿时长

13 weeks

期刊介绍： Natural Products and Bioprospecting serves as an international forum for essential research on natural products and focuses on, but is not limited to, the following aspects: Natural products: isolation and structure elucidation Natural products: synthesis Biological evaluation of biologically active natural products Bioorganic and medicinal chemistry Biosynthesis and microbiological transformation Fermentation and plant tissue cultures Bioprospecting of natural products from natural resources All research articles published in this journal have undergone rigorous peer review. In addition to original research articles, Natural Products and Bioprospecting publishes reviews and short communications, aiming to rapidly disseminate the research results of timely interest, and comprehensive reviews of emerging topics in all the areas of natural products. It is also an open access journal, which provides free access to its articles to anyone, anywhere.