Skin treatment with non-thermal plasma modulates the immune system through miR-223-3p and its target genes.

IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
RNA Biology Pub Date : 2024-01-01 Epub Date: 2024-06-03 DOI:10.1080/15476286.2024.2361571
Annika Engel, Nicole Ludwig, Friederike Grandke, Viktoria Wagner, Fabian Kern, Tobias Fehlmann, Georges P Schmartz, Ernesto Aparicio-Puerta, Dominic Henn, Barbara Walch-Rückheim, Matthias Hannig, Stefan Rupf, Eckart Meese, Matthias W Laschke, Andreas Keller
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引用次数: 0

Abstract

Non-thermal plasma, a partially ionized gas, holds significant potential for clinical applications, including wound-healing support, oral therapies, and anti-tumour treatments. While its applications showed promising outcomes, the underlying molecular mechanisms remain incompletely understood. We thus apply non-thermal plasma to mouse auricular skin and conducted non-coding RNA sequencing, as well as single-cell blood sequencing. In a time-series analysis (five timepoints spanning 2 hours), we compare the expression of microRNAs in the plasma-treated left ears to the unexposed right ears of the same mice as well as to the ears of unexposed control mice. Our findings indicate specific effects in the treated ears for a set of five miRNAs: mmu-miR-144-5p, mmu-miR-144-3p, mmu-miR-142a-5p, mmu-miR-223-3p, and mmu-miR-451a. Interestingly, mmu-miR-223-3p also exhibits an increase over time in the right non-treated ear of the exposed mice, suggesting systemic effects. Notably, this miRNA, along with mmu-miR-142a-5p and mmu-miR-144-3p, regulates genes and pathways associated with wound healing and tissue regeneration (namely ErbB, FoxO, Hippo, and PI3K-Akt signalling). This co-regulation is particularly remarkable considering the significant seed dissimilarities among the miRNAs. Finally, single-cell sequencing of PBMCs reveals the downregulation of 12 from 15 target genes in B-cells, Cd4+ and Cd8+ T-cells. Collectively, our data provide evidence for a systemic effect of non-thermal plasma.

用非热等离子体进行皮肤治疗可通过 miR-223-3p 及其靶基因调节免疫系统。
非热等离子体是一种部分电离的气体,在伤口愈合支持、口腔治疗和抗肿瘤治疗等临床应用方面具有巨大潜力。虽然非热等离子体的应用显示出良好的效果,但人们对其潜在的分子机制仍不甚了解。因此,我们将非热等离子体应用于小鼠耳廓皮肤,并进行了非编码 RNA 测序和单细胞血液测序。通过时间序列分析(5 个时间点,时间跨度为 2 小时),我们比较了经血浆处理的小鼠左耳与未接触血浆的小鼠右耳以及未接触血浆的对照组小鼠耳朵中 microRNA 的表达情况。我们的研究结果表明,经处理的小鼠耳朵中的五种 miRNA 具有特异性影响:mmu-miR-144-5p、mmu-miR-144-3p、mmu-miR-142a-5p、mmu-miR-223-3p 和 mmu-miR-451a。有趣的是,随着时间的推移,暴露小鼠未接受治疗的右耳中的 mmu-miR-223-3p 也会增加,这表明会产生系统性影响。值得注意的是,这种 miRNA 与 mmu-miR-142a-5p 和 mmu-miR-144-3p 一起调节与伤口愈合和组织再生相关的基因和通路(即 ErbB、FoxO、Hippo 和 PI3K-Akt 信号)。考虑到 miRNA 之间存在显著的种子差异,这种共同调控尤其引人注目。最后,PBMCs 的单细胞测序显示,在 B 细胞、Cd4+ 和 Cd8+ T 细胞中,15 个目标基因中有 12 个被下调。总之,我们的数据为非热等离子体的系统效应提供了证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
RNA Biology
RNA Biology 生物-生化与分子生物学
CiteScore
8.60
自引率
0.00%
发文量
82
审稿时长
1 months
期刊介绍: RNA has played a central role in all cellular processes since the beginning of life: decoding the genome, regulating gene expression, mediating molecular interactions, catalyzing chemical reactions. RNA Biology, as a leading journal in the field, provides a platform for presenting and discussing cutting-edge RNA research. RNA Biology brings together a multidisciplinary community of scientists working in the areas of: Transcription and splicing Post-transcriptional regulation of gene expression Non-coding RNAs RNA localization Translation and catalysis by RNA Structural biology Bioinformatics RNA in disease and therapy
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