Molecular Characterization and Genetic Relationships in Different Mint (Mentha L.) Species with ISSR Marker Technique

IF 0.5 4区 生物学 Q4 BIOLOGY
Civan Çelik, Nasir Ahmad Seraj, Selma Yasak, Yaşar Karakurt, İsa Telci, Emre Sevindik
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Abstract

In the study, it was aimed to determine the molecular characterization and genetic relationships of Mentha species obtained from different countries and regions at the molecular level and to ensure the sustainability of these species. For this purpose, ISSR marker technique, which is effective in detecting polymorphism and genetic variation within and between Mentha species, was used. In the study a total of 460 allelic bands were detected, and all bands were found to be 100% polymorphic. The maximum number of bands was produced by the ISSR7 primer (25), but the minimum number of bands was produced by the ISSR12 primer (13). The highest PIC (Polymorphism Information Content) value was detected in the ISSR3 primer (0.521) but the lowest PIC value was determined in the 903 primer (0.203). Molecular datas were analyzed using the NTSYSpc 2.02 software and it was determined that the mint species were divided into two main groups. In the results obtained, it was determined that there was no relationship between the genetic diversity and geographical origins, and the populations from different geographical regions were in the same clusters. In the study, it was concluded that there was a significant molecular diversity among the species, and that some Mentha clones, which are defined by morphological features and still remain taxonomically uncertain, were belong to which species at the molecular level. Therefore, it is insufficient to classify species with morphochemical, morphological and phenotypic characteristics.

Abstract Image

利用 ISSR 标记技术确定不同薄荷(Mentha L.)品种的分子特征和遗传关系
摘要 本研究旨在从分子水平上确定从不同国家和地区获得的薄荷物种的分子特征和遗传关系,并确保这些物种的可持续性。为此,研究人员采用了 ISSR 标记技术,该技术可有效检测薄荷物种内部和物种之间的多态性和遗传变异。研究共检测到 460 条等位基因条带,发现所有条带都具有 100% 的多态性。ISSR7 引物产生的条带数最多(25 条),而 ISSR12 引物产生的条带数最少(13 条)。ISSR3 引物的多态性信息含量(PIC)值最高(0.521),而 903 引物的 PIC 值最低(0.203)。使用 NTSYSpc 2.02 软件对分子数据进行了分析,结果表明薄荷物种被分为两大类。研究结果表明,遗传多样性与地理起源之间没有关系,来自不同地理区域的种群属于同一群组。研究得出的结论是,物种之间存在显著的分子多样性,一些根据形态特征确定的薄荷克隆在分类学上仍不确定,但在分子水平上却属于哪个物种。因此,以形态化学特征、形态特征和表型特征来划分物种是不够的。
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来源期刊
Biology Bulletin
Biology Bulletin 生物-生物学
CiteScore
0.70
自引率
20.00%
发文量
84
审稿时长
4-8 weeks
期刊介绍: Biology Bulletin (Izvestiya Rossiiskoi Akademii Nauk – Seriya Biologicheskaya) is an interdisciplinary journal of general biology. It focuses on fundamental studies in the fields of cell biology, biochemistry, zoology, botany, physiology, and ecology. This journal publishes current materials of experimental studies and surveys on current problems in general biology. It also publishes information on scientific conferences and new books in the fields of general biology.
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