Aryl hydrocarbon receptor (AhR) is regulated by hyperoxia in premature infants.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-12-01 Epub Date: 2024-05-30 DOI:10.1080/14767058.2024.2349179

Xi Yang, Xia Wang, Wenbin Dong

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引用次数: 0

Abstract

Objective: To investigate whether aryl hydrocarbon receptor (AhR) is involved in hyperoxia-mediated oxidative stress by observing the relationship between AhR and reactive oxygen species (ROS) in peripheral blood mononuclear cells (PBMCs) after oxygen exposure in premature infants.

Methods: After 48 h of oxygen inhalation at different concentrations, discarded peripheral blood was collected to separate PBMCs and plasma. ROS were labeled with MitoSOX^TM Red and detected by fluorescence microscopy in PBMCs. The level of MDA in plasma was detected by thiobarbituric acid colorimetry, the level of MCP-1 in plasma was detected by enzyme-linked immunosorbent assay (ELISA), the localization of AhR was detected by immunofluorescence, and the level of AhR expression in PBMCs was detected by Western blotting.

Results: As the volume fraction of inspired oxygen increased, compared with those in the air control group, the levels of ROS, MDA in plasma, and MCP-1 in plasma increased gradually in the low concentration oxygen group, medium concentration oxygen group and high concentration oxygen group. The cytoplasm-nuclear translocation rate of AhR gradually increased, and the expression level of AhR gradually decreased. The levels of ROS in PBMCs, MDA in the plasma and MCP-1 in the plasma of premature infants were positively correlated with the cytoplasm-nuclear translocation rate of AhR but negatively correlated with the level of AhR expression.

Conclusion: Aryl hydrocarbon receptor (AhR) is regulated by hyperoxia in premature infants.

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早产儿的芳基烃受体（AhR）受高氧调节。

目的通过观察早产儿暴露于氧气后外周血单核细胞（PBMCs）中的芳基烃受体（AhR）与活性氧（ROS）之间的关系，研究芳基烃受体（AhR）是否参与高氧介导的氧化应激：方法：吸入不同浓度的氧气 48 小时后，收集弃血，分离外周血单核细胞和血浆。用 MitoSOXTM Red 标记 ROS，并用荧光显微镜检测 PBMCs。硫代巴比妥酸比色法检测血浆中 MDA 的水平，酶联免疫吸附法（ELISA）检测血浆中 MCP-1 的水平，免疫荧光法检测 AhR 的定位，Western 印迹法检测 AhR 在 PBMCs 中的表达水平：结果：与空气对照组相比，随着吸入氧体积分数的增加，低浓度氧组、中浓度氧组和高浓度氧组的 ROS、血浆中 MDA 和血浆中 MCP-1 的水平逐渐升高。AhR 的胞浆-核易位率逐渐升高，表达水平逐渐降低。早产儿 PBMC 中的 ROS 水平、血浆中的 MDA 水平和血浆中的 MCP-1 水平与 AhR 的胞浆核转位率呈正相关，但与 AhR 的表达水平呈负相关：结论：早产儿的芳香烃受体（AhR）受高氧调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.