Catalytic physiological amyloids.

4区 生物学 Q3 Biochemistry, Genetics and Molecular Biology
Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-02-27 DOI:10.1016/bs.mie.2024.01.014
Elad Arad, Raz Jelinek
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引用次数: 0

Abstract

Amyloid fibrils have been identified in many protein systems, mostly linked to progression and cytotoxicity in neurodegenerative diseases and other pathologies, but have also been observed in normal physiological systems. A growing body of work has shown that amyloid fibrils can catalyze chemical reactions. Most studies have focused on catalysis by de-novo synthetic amyloid-like peptides; however, recent studies reveal that physiological, native amyloids are catalytic as well. Here, we discuss methodologies and major experimental aspects pertaining to physiological catalytic amyloids. We highlight analyzes of kinetic parameters related to the catalytic activities of amyloid fibrils, structure-function considerations, characterization of the catalytic active sites, and deciphering of catalytic mechanisms.

催化生理淀粉样蛋白。
在许多蛋白质系统中都发现了淀粉样纤维,主要与神经退行性疾病和其他病症的进展和细胞毒性有关,但在正常生理系统中也观察到了淀粉样纤维。越来越多的研究表明,淀粉样蛋白纤维可以催化化学反应。大多数研究都集中在新合成的淀粉样肽的催化作用上;然而,最近的研究发现,生理性的原生淀粉样肽也具有催化作用。在此,我们将讨论有关生理性催化淀粉样肽的方法和主要实验方面。我们重点分析了与淀粉样纤维催化活性有关的动力学参数、结构-功能考虑因素、催化活性位点的特征以及催化机制的破译。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Methods in enzymology
Methods in enzymology 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
308
审稿时长
3-6 weeks
期刊介绍: The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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