Comprehensive Quality Evaluation of Danggui-Jianzhong Decoction by Fingerprint Analysis, Multi-Component Quantitation and UPLC-Q-TOF-MS.

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of chromatographic science Pub Date : 2024-08-20 DOI:10.1093/chromsci/bmae034

Lanyi Huang, Qing Liu, Wenfang Zhang, Bishan Lin, Yongjian Gao, Hong Deng, Shu Zhang

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引用次数: 0

Abstract

Danggui-Jianzhong decoction (DGJZ) is a famous classical traditional Chinese medicine formula, which ingredients are complex and the quality is difficult to control. Our study aimed to identify the overall chemical profile of DGJZ qualitatively by ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and UPLC. A total of 77 components, including terpenoids, flavonoids, phenolic acids, gingerols and other components, were firstly detected and characterized by UPLC-Q-TOF-MS and 18 peaks marked after analyzing the UPLC fingerprint. Finally, paeoniflorin, liquiritin, ferulic acid, cinnamic acid, glycyrrhizic acid and 6-gingerol were quantified, which was validated in terms of linearity, precision, accuracy, repeatability and recovery. Taken together, the chemical constitutes of DGJZ were systematically identified and a reliable quantitative method coupled with fingerprint analysis was successfully employed for evaluating the holistic quality, which will provide a robust foundation for the quality control of DGJZ.

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利用指纹图谱分析、多组分定量和UPLC-Q-TOF-MS对当归建中煎剂进行综合质量评价

当归建中汤（Danggui-Jianzhong decoction，DGJZ）是一种著名的经典中药配方，其成分复杂，质量难以控制。我们的研究旨在采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）和超高效液相色谱法（UPLC）对当归建中煎剂的整体化学成分进行定性鉴定。首先利用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）对萜类、黄酮类、酚酸类、姜辣素类等 77 种成分进行了检测和定性，并通过超高效液相色谱指纹图谱分析标记了 18 个峰。最后对芍药苷、枸杞苷、阿魏酸、肉桂酸、甘草酸和 6-姜酚进行了定量分析，并在线性、精密度、准确度、重复性和回收率等方面进行了验证。综上所述，该方法系统地鉴定了DGJZ的化学成分，并采用可靠的定量方法结合指纹图谱分析成功地评价了DGJZ的整体质量，为DGJZ的质量控制奠定了坚实的基础。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.