Effects of Splenectomy on Natural Killer Cell Levels in β-Thalassemia Major Patients

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2024-05-30 DOI:10.1002/jcla.25046

Ayşegül Uğur Kurtoğlu, Sevcan Uğur, Mesut Göçer, Erdal Kurtoğlu

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引用次数: 0

Abstract

Aim

In this study, we investigated how splenectomy affects natural killer (NK) cell levels in patients with β-thalassemia major (β-TM).

Materials and Methods

Seventy patients with β-TM (38 splenectomized and 32 nonsplenectomized) and 25 healthy controls were included in this study. The hemogram parameters, ferritin, T lymphocyte, T-helper cell, T-suppressor cell, and NK cell numbers, were measured.

Results

The T lymphocyte (CD3⁺) level was found to be significantly higher in the patient group (p < 0.05). CD3⁺/CD4⁺ T lymphocytes were detected to be significantly higher in the patient group (p < 0.05). Although the CD3⁺/CD4⁺ T lymphocyte level was significantly higher in the nonsplenectomy group (p < 0.05), this was not the case in the splenectomy group. When the patient and control groups were compared, no significant difference was detected regarding CD3⁺/CD8⁺ T lymphocyte levels. CD3⁻/CD16⁺CD56⁺ NK cell level was found to be significantly lower only in the splenectomy group than in the control group (p < 0.05). We found that there was a significant negative correlation between serum ferritin levels and both total lymphocyte (r = −0.617) and CD3⁺ lymphocyte (r = −0.718) levels in the control group (p < 0.05). A significant negative correlation was detected between serum ferritin levels and CD3⁻/CD16⁺CD56⁺ NK cell levels in the patient group (r = −0.410) (p < 0.05).

Conclusion

Splenectomy reduces NK cell levels in patients with β-TM. The negative relationship between ferritin levels and NK cells indicates that ferritin levels should be kept under control in patients with β-TM.

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脾切除术对重型β地中海贫血患者自然杀伤细胞水平的影响

目的：本研究探讨了脾切除术如何影响重型β地中海贫血（β-TM）患者的自然杀伤（NK）细胞水平：研究对象包括 70 名重型地中海贫血患者（38 名脾切除患者和 32 名非脾切除患者）和 25 名健康对照者。研究人员测量了血象参数、铁蛋白、T淋巴细胞、T辅助细胞、T抑制细胞和NK细胞的数量：发现患者组的 T 淋巴细胞（CD3+）水平明显高于非脾切除组（p +/CD4+ T 淋巴细胞水平明显高于非脾切除组（p +/CD8+ T 淋巴细胞水平。发现只有脾切除组的 CD3-/CD16+CD56+ NK 细胞水平明显低于对照组（对照组的 p + 淋巴细胞水平（r = -0.718），患者组的 p -/CD16+CD56+ NK 细胞水平（r = -0.410）（p 结论：脾切除会降低 NK 细胞水平：脾切除可降低β-TM患者的NK细胞水平。铁蛋白水平与 NK 细胞之间的负相关表明，β-TM 患者应控制铁蛋白水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.