Overexpression of GmWRKY17, a class IIb WRKY transcription factor from Glycine max, enhances drought tolerance in Arabidopsis plants

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Plant Growth Regulation Pub Date : 2024-05-27 DOI:10.1007/s10725-024-01157-7

Yi Liu, Yueping Cao

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Abstract

Drought is one of the abiotic stresses affecting agricultural production. WRKY transcription factors have important functions in response to drought. However, functions of only a few WRKY transcription factors are understood in soybean. Here, GmWRKY17 was cloned from soybean and shown to bind the W-box sequence. GmWRKY17 was tissue-specific and induced by drought. Overexpression of GmWRKY17 was found to decrease abscisic acid (ABA) sensitivity and increase drought tolerance in Arabidopsis thaliana. GmWRKY17 improved germination rate, leaf opening and greening, root length and chlorophyll content under osmotic stress and drought. GmWRKY17 reduced malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) levels under drought. GmWRKY17 promoted stomatal closure after treatment with 10% polyethylene glycol 6000 (PEG6000). Overexpression of GmWRKY17 increased superoxide dismutase (SOD) activity and catalase (CAT) activity, and GmWRKY17 promoted the expression of AtSOD1 and AtCAT1 under drought. Moreover, GmWRKY17 increased proline content and enhanced the expression of AtP5CS1, and GmWRKY17 up-regulated stress-related genes under drought stress. All results showed that overexpression of GmWRKY17 improved drought tolerance, and GmWRKY17 may regulate drought stress by enhancing antioxidant activity and upregulating stress-related genes in Arabidopsis thaliana.

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过表达拟南芥植物中的 IIb 类 WRKY 转录因子 GmWRKY17 可增强其耐旱性

干旱是影响农业生产的非生物胁迫之一。WRKY 转录因子在应对干旱方面具有重要功能。然而，人们只了解大豆中少数 WRKY 转录因子的功能。本文克隆了大豆中的 GmWRKY17，并证明其能与 W-box 序列结合。GmWRKY17 具有组织特异性，并受干旱诱导。过表达 GmWRKY17 能降低拟南芥对脱落酸（ABA）的敏感性并提高其耐旱性。GmWRKY17 提高了拟南芥在渗透胁迫和干旱条件下的发芽率、叶片张开和变绿、根长和叶绿素含量。GmWRKY17 降低了干旱条件下丙二醛（MDA）和过氧化氢（H2O2）的水平。GmWRKY17能促进10%聚乙二醇6000（PEG6000）处理后的气孔关闭。过表达 GmWRKY17 能提高超氧化物歧化酶（SOD）活性和过氧化氢酶（CAT）活性，并且 GmWRKY17 能促进 AtSOD1 和 AtCAT1 在干旱条件下的表达。此外，GmWRKY17 增加了脯氨酸含量，提高了 AtP5CS1 的表达，GmWRKY17 还上调了干旱胁迫下的胁迫相关基因。所有结果表明，GmWRKY17的过表达提高了拟南芥的抗旱性，GmWRKY17可能通过增强抗氧化活性和上调胁迫相关基因来调控干旱胁迫。

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来源期刊

Plant Growth Regulation 生物-植物科学

CiteScore

6.90

自引率

9.50%

发文量

139

审稿时长

4.5 months

期刊介绍： Plant Growth Regulation is an international journal publishing original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research using hormonal, physiological, environmental, genetical, biophysical, developmental or molecular approaches to the study of plant growth regulation. Emphasis is placed on papers presenting the results of original research. Occasional reviews on important topics will also be welcome. All contributions must be in English.