CRISPRi functional genomics in bacteria and its application to medical and industrial research.

IF 8 1区生物学 Q1 MICROBIOLOGY

Microbiology and Molecular Biology Reviews Pub Date : 2024-06-27 Epub Date: 2024-05-29 DOI:10.1128/mmbr.00170-22

Amy L Enright, William J Heelan, Ryan D Ward, Jason M Peters

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Abstract

SUMMARYFunctional genomics is the use of systematic gene perturbation approaches to determine the contributions of genes under conditions of interest. Although functional genomic strategies have been used in bacteria for decades, recent studies have taken advantage of CRISPR (clustered regularly interspaced short palindromic repeats) technologies, such as CRISPRi (CRISPR interference), that are capable of precisely modulating expression of all genes in the genome. Here, we discuss and review the use of CRISPRi and related technologies for bacterial functional genomics. We discuss the strengths and weaknesses of CRISPRi as well as design considerations for CRISPRi genetic screens. We also review examples of how CRISPRi screens have defined relevant genetic targets for medical and industrial applications. Finally, we outline a few of the many possible directions that could be pursued using CRISPR-based functional genomics in bacteria. Our view is that the most exciting screens and discoveries are yet to come.

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细菌的 CRISPRi 功能基因组学及其在医学和工业研究中的应用。

摘要功能基因组学是利用系统的基因扰乱方法来确定基因在相关条件下的贡献。虽然功能基因组学策略在细菌中已经应用了几十年，但最近的研究利用了CRISPR（簇状规则间隔短回文重复序列）技术，如CRISPRi（CRISPR干扰），这种技术能够精确调节基因组中所有基因的表达。在此，我们讨论并回顾了 CRISPRi 和相关技术在细菌功能基因组学中的应用。我们讨论了 CRISPRi 的优缺点以及 CRISPRi 基因筛选的设计注意事项。我们还回顾了 CRISPRi 筛选如何为医疗和工业应用确定相关基因靶标的实例。最后，我们概述了利用基于 CRISPR 技术的细菌功能基因组学研究的几个可能方向。我们认为，最激动人心的筛选和发现还在后面。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology and Molecular Biology Reviews 生物-微生物学

CiteScore

18.80

自引率

0.80%

发文量

期刊介绍： Microbiology and Molecular Biology Reviews (MMBR), a journal that explores the significance and interrelationships of recent discoveries in various microbiology fields, publishes review articles that help both specialists and nonspecialists understand and apply the latest findings in their own research. MMBR covers a wide range of topics in microbiology, including microbial ecology, evolution, parasitology, biotechnology, and immunology. The journal caters to scientists with diverse interests in all areas of microbial science and encompasses viruses, bacteria, archaea, fungi, unicellular eukaryotes, and microbial parasites. MMBR primarily publishes authoritative and critical reviews that push the boundaries of knowledge, appealing to both specialists and generalists. The journal often includes descriptive figures and tables to enhance understanding. Indexed/Abstracted in various databases such as Agricola, BIOSIS Previews, CAB Abstracts, Cambridge Scientific Abstracts, Chemical Abstracts Service, Current Contents- Life Sciences, EMBASE, Food Science and Technology Abstracts, Illustrata, MEDLINE, Science Citation Index Expanded (Web of Science), Summon, and Scopus, among others.

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