Assessing the effectiveness and mechanisms of Fe/PS, Fe/H2O2, and O3 treatment for water disinfection: Spotlight on VBNC bacteria

IF 5.9 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Zheng Qi , Zaihui Huang , Chunguang Liu
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引用次数: 0

Abstract

Advanced oxidation processes (AOPs) exhibit significant potential for water disinfection due to their generation of large quantities of highly oxidizing free radicals. However, the neglect of viable but nonculturable (VBNC) cells obscures their true disinfection efficacy and potential environmental health risks. Therefore, the study evaluated the disinfection effectiveness and mechanisms of typical AOPs, including Fe/H2O2, Fe/persulfate (PS), and O3, from the perspective of the production of VBNC bacteria. The results indicate that Fe/PS exhibits the strongest bacterial inactivation rate (99.94%), and the cells lose their ability to reactivate. Fe/H2O2 and O3 induce more cells to enter the VBNC state compared to Fe/PS. Moreover, different AOPs result in varying levels of free radical production and utilization efficiency, with SO4•− and O3 exhibiting greater selectivity in deactivating bacteria compared to HO. The inhibition of VBNC bacteria production by Fe/PS treatment may be attributed to the combined action of HO and SO4•− on microorganisms, leading to oxidative stress and metabolic disruption in bacteria through the inhibition of biofilm formation and aminoacyl-tRNA biosynthesis (p < 0.05), thereby causing direct bacterial death rather than entry into the VBNC state. In contrast, Fe/H2O2 and O3 result in the upregulation of the metabolism of alanine, aspartate, and glutamate, as well as styrene degradation capacity by the bacteria, leading to the production of more VBNC bacteria. Overall, the study offers insights into mitigating potential biological risks in water disinfection and developing environmentally friendly and efficient disinfection technologies.

Abstract Image

评估Fe/PS、Fe/H2O2和O3处理水消毒的效果和机制:聚焦 VBNC 细菌
高级氧化工艺(AOPs)可产生大量高度氧化自由基,因此在水消毒方面具有巨大潜力。然而,由于忽略了可存活但不可培养的细胞(VBNC),因此掩盖了其真正的消毒功效和潜在的环境健康风险。因此,本研究从 VBNC 细菌产生的角度评估了典型 AOP 的消毒效果和机制,包括 Fe/H2O2、Fe/过硫酸盐(PS)和 O3。结果表明,Fe/PS 的细菌灭活率最高(99.94%),细胞失去了再活化能力。与 Fe/PS 相比,Fe/H2O2 和 O3 能诱导更多的细胞进入 VBNC 状态。此外,不同的 AOP 会导致不同程度的自由基产生和利用效率,与 HO- 相比,SO4- 和 O3 在使细菌失活方面表现出更大的选择性。Fe/PS 处理对 VBNC 细菌产生的抑制作用可能是由于 HO- 和 SO4- 对微生物的联合作用,通过抑制生物膜的形成和氨基酰-tRNA 的生物合成(p < 0.05),导致细菌氧化应激和代谢紊乱,从而直接导致细菌死亡,而不是进入 VBNC 状态。与此相反,Fe/H2O2 和 O3 会导致细菌的丙氨酸、天冬氨酸和谷氨酸代谢以及苯乙烯降解能力上调,从而产生更多的 VBNC 细菌。总之,这项研究为减轻水消毒中潜在的生物风险和开发环境友好型高效消毒技术提供了启示。
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来源期刊
Journal of Environmental Sciences-china
Journal of Environmental Sciences-china 环境科学-环境科学
CiteScore
13.70
自引率
0.00%
发文量
6354
审稿时长
2.6 months
期刊介绍: The Journal of Environmental Sciences is an international journal started in 1989. The journal is devoted to publish original, peer-reviewed research papers on main aspects of environmental sciences, such as environmental chemistry, environmental biology, ecology, geosciences and environmental physics. Appropriate subjects include basic and applied research on atmospheric, terrestrial and aquatic environments, pollution control and abatement technology, conservation of natural resources, environmental health and toxicology. Announcements of international environmental science meetings and other recent information are also included.
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