Aptamer-based electrochemical nanobiosensor for research and monitoring of multiple sclerosis in mice models

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2024-05-19 DOI:10.1016/j.bioelechem.2024.108744

Marina Serin , Pınar Kara

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引用次数: 0

Abstract

Multiple sclerosis (MS) is a severe progressive autoimmune-inflammatory, demyelinating process in the central nervous system (CNS) with heterogeneous neurological symptoms appearing as a consequence of myelin break down. Myelin basic protein (MBP) makes up to 30 % of the CNS myelin [1] and it is known to be released into the cerebrospinal fluid (CSF) as a bioindicator of MS. Autoimmune encephalomyelitis (EAE) is a mice model of MS widely used for research and development of new treatments [2]. Herein, MBP specific aptamer developed for possible therapeutic purposes in mouse model [3] was applied as a bioreceptor for MBP recognition. A nanobiosensor for MBP detection and monitoring was developed by using graphene oxide (GO) nanoparticles integrated onto the screen-printed carbon electrodes (SPCE) and aptamer immobilized to create a bioactive layer on the sensor surface for MBP binding. The measurements were carried out using electrochemical impedance spectrometry (EIS). Validation studies were carried out in a biological matrix (artificial CSF) containing MBP, and MSA. The aptasensor had LOD in artificial CSF 0.01 ng/mL and showed its usability in the concentration range of 0.01 … 64 ng/mL.

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用于研究和监测多发性硬化症小鼠模型的基于色胺的电化学纳米生物传感器

多发性硬化症（MS）是中枢神经系统（CNS）中一种严重的进行性自身免疫炎症脱髓鞘过程，由于髓鞘破坏而出现各种神经症状。髓鞘碱性蛋白（MBP）占中枢神经系统髓鞘的 30%[1]，它作为多发性硬化症的生物指标释放到脑脊液（CSF）中。自身免疫性脑脊髓炎（EAE）是一种多发性硬化症小鼠模型，被广泛用于研究和开发新的治疗方法[2]。在此，为小鼠模型可能的治疗目的而开发的 MBP 特异性适配体[3]被用作识别 MBP 的生物受体。利用集成在丝网印刷碳电极（SPCE）上的氧化石墨烯（GO）纳米颗粒和固定在传感器表面以形成生物活性层来结合 MBP 的适配体，开发了一种用于检测和监测 MBP 的纳米生物传感器。测量采用电化学阻抗谱法（EIS）进行。在含有 MBP 和 MSA 的生物基质（人工 CSF）中进行了验证研究。该适配传感器在人工 CSF 中的检出限为 0.01 纳克/毫升，在 0.01 ... 64 纳克/毫升的浓度范围内显示出其可用性。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.