Molecular characterization of Ehrlichia canis from naturally infected dogs reveals a novel Asiatic-lineage and co-circulation of multiple lineages in India

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2024-05-23 DOI:10.1016/j.rvsc.2024.105311

Aravindh Babu R. Parthiban , Azhahianambi Palavesam , Sharmila Srinivasan , Arya Mohanan , Srikanta Ghosh , Tirumurugaan Krishnaswamy Gopalan

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引用次数: 0

Abstract

Canine monocytic ehrlichiosis caused by Ehrlichia canis is an important rickettsial pathogen of dogs transmitted by Rhipicephalus sanguineus sensu lato ticks in India. Globally, molecular characterization of E. canis is done using different E. canis gene targets. This study aimed to characterize genetic diversity of uncultured Ehrlichia species from dogs by 16S rRNA and partial gp200 gene (termed as p43 region) sequences data. Phylogeny based on 16S rRNA gene did not reveal any region-specific lineages. The phylogeny based on 5′ region of E. canis gp200 gene (termed as p43 region) revealed four major clusters (A, B, C and D) and the Indian isolates fall under clusters A and B. Cluster A is characterized by an insertion of unique 141 bp tandem repeat sequence. Similar tandem repeat sequence was found in one of the E canis isolates from east-Asia, suggesting a possible divergence within this species. The study shows evidence for divergence of a new lineage within E. canis. The location of this insertion at the ‘ankyrin repeat domains' containing region is suggestive of its possible role in modulation of host responses.

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从自然感染的狗身上提取的犬艾氏杆菌的分子特征揭示了一个新的亚洲系和多个系在印度的共同流行情况

犬单核细胞埃希氏菌引起的犬单核细胞埃希氏病是一种重要的立克次体病原体，在印度由Rhipicephalus sanguineus sensu lato蜱传播。在全球范围内，犬艾里希氏菌的分子特征描述是通过不同的犬艾里希氏菌基因靶标来完成的。本研究旨在通过 16S rRNA 和部分 gp200 基因（称为 p43 区域）序列数据来描述狗身上未培养的埃利希氏菌的遗传多样性。基于 16S rRNA 基因的系统进化未发现任何区域特异性系谱。基于犬大肠杆菌 gp200 基因 5′区（称为 p43 区）的系统进化发现了四个主要簇（A、B、C 和 D），印度分离物属于簇 A 和簇 B。在东亚的一个犬大肠杆菌分离物中也发现了类似的串联重复序列，这表明该物种内部可能存在分化。该研究表明犬大肠杆菌内部出现了新的分化。这种插入位于含有 "ankyrin重复域 "的区域，表明它可能在调节宿主反应中发挥作用。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.