Calcium Signal Analysis in the Zebrafish Heart via Phase Matching of the Cardiac Cycle

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-20 DOI:10.21769/BioProtoc.4989

Raymond Zhang, Julien Vermot, Riccardo Gherardi, Hajime Fukui, Renee Chow

引用次数: 0

Abstract

Calcium signalling in the endocardium is critical for heart valve development. Calcium ion pulses in the endocardium are generated in response to mechanical forces due to blood flow and can be visualised in the beating zebrafish heart using a genetically encoded calcium indicator such as GCaMP7a. Analysing these pulses is challenging because of the rapid movement of the heart during heartbeat. This protocol outlines an imaging analysis method used to phase-match the cardiac cycle in single z-slice movies of the beating heart, allowing easy measurement of the calcium signal. Key features • Software to synchronise and analyse frames from movies of the beating heart corresponding to a user-defined phase of the cardiac cycle. • Software to measure the fluorescence intensity of the beating heart corresponding to a user-defined region of interest.

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通过心动周期相位匹配分析斑马鱼心脏中的钙信号

心内膜中的钙信号对心脏瓣膜的发育至关重要。心内膜中的钙离子脉冲是对血流产生的机械力的反应，可通过基因编码的钙指示剂（如 GCaMP7a）在跳动的斑马鱼心脏中观察到。由于心脏在搏动过程中快速运动，因此分析这些脉冲具有挑战性。本方案概述了一种成像分析方法，用于在心脏跳动的单个 Z 切片影片中对心脏周期进行相位匹配，从而轻松测量钙信号。主要功能 - 用软件同步和分析与用户定义的心动周期相位相对应的心脏跳动影片帧。- 软件可测量与用户定义的感兴趣区域相对应的心脏跳动荧光强度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.