DECOMPOSITION OF THE ABSORPTION SPECTRUM ACETONE EXTRACT OF MICROALGAE ARTHROSPIRA (SPIRULINA) PLATENSIS NORTH. GEITL.

Abstract

We have described the absorption spectra of acetone extract of the microalga Arthrospira (Spirulina) platensis by mathematical models of carotenoids and chlorophylls in the range from 400 to 700 nm. The obtained values of pigment concentrations agree well with standard methods. A model of the absorption spectrum of acetone extract of A. platensis can be used to determine the concentration of chlorophyll a and carotenoids. The absence of chlorophyll b contribution to the total absorption spectrum was shown. The method of mathematical separation of acetone extract absorption spectrum in Microsoft Excel program was implemented. Calculations performing approximations of pigment concentrations by minimizing the sum of squares of deviations between the true absorption spectrum and its mathematical model have been performed using the spreadsheet processor with the help of the solution search tool.