Uji Aktivitas Anti-Inflamasi Ekstrak Sabut Kelapa (Cocos nucifera L.) dengan Metode Stabilisasi Membran Sel Darah Merah

e-GiGi Pub Date : 2024-05-22 DOI:10.35790/eg.v13i1.55338

Damajanty H. C. Pangemanan Pangemanan, Ni Wayan Mariati, Angelin L. Rantetondok

{"title":"Uji Aktivitas Anti-Inflamasi Ekstrak Sabut Kelapa (Cocos nucifera L.) dengan Metode Stabilisasi Membran Sel Darah Merah","authors":"Damajanty H. C. Pangemanan Pangemanan, Ni Wayan Mariati, Angelin L. Rantetondok","doi":"10.35790/eg.v13i1.55338","DOIUrl":null,"url":null,"abstract":"Abstract: Dental and oral care often causes injuries that can trigger an inflammatory reaction associated with feeling of discomfort. Drugs that can be given for the treatment of inflammation, namely corticosteroids of the glucocorticoid group and non-steroidal anti-inflammatories (NSAIDs). However, these drugs have side effects, therefore, alternatives with minimal toxicity that can be found in plants are preferrable. Coconut coir has the potential to be an anti-inflammatory drug because it contains flavonoids, tannins and saponins. This study aimed to determine the anti-inflammatory activity of coconut coir extract at concentrations of 50, 100, 150, 500, and 1000 ppm. This was a pure experimental study with a post-test-only control design using blood of rats weighing above 250 grams taken through the retro-orbital sinus. The samples were divided into seven groups, namely 50, 100, 150, 500, 1000 ppm, positive control, and negative control. The results showed that coconut coir extract contained alkaloids, flavonoids, tannins, saponins, and terpenoids. The percentages of inhibition of hemolysis were obtained at 50, 100, 150, 500, and 1000 ppm, namely 14.73%, 24.60%, 38.86%, 43.11%, and 50.39%. In conclusion, coconut coir extract (Cocos nucifera L.) has anti-inflammatory activity. Extract concentration of 1000 ppm has the highest anti-inflammatory activity.\nKeywords: inflammation; coconut coir extract; stabilization of red blood cell membranes\n \nAbstrak: Perawatan gigi dan mulut tidak jarang menimbulkan perlukaan yang dapat memicu reaksi inflamasi disertai rasa tidak nyaman. Golongan obat yang dapat diberikan untuk pengobatan inflamasi, yaitu kortikosteroid golongan glukokortikoid dan anti-inflamasi non-steroid (AINS). Namun, obat-obat tersebut memiliki efek samping sehingga dibutuhkan alternatif dengan toksisitas minimal yang dapat ditemukan pada tanaman. Sabut kelapa berpotensi menjadi obat anti-inflamasi karena mengandung flavonoid, tanin, dan saponin. Penelitian ini bertujuan untuk mengetahui aktivitas anti-inflamasi ekstrak sabut kelapa konsentrasi 50, 100, 150, 500, dan 1000 ppm. Penelitian ini merupakan eksperimental murni dengan post-test only control design menggunakan darah tikus yang diambil melalui sinus retro-orbitalis. Kriteria tikus ialah berat di atas 250 gram, yang dibagi menjadi tujuh kelompok, yaitu 50, 100, 150, 500, 1000 ppm, kontrol positif, dan kontrol negatif. Hasil penelitian mendapatkan bahwa ekstrak sabut kelapa mengandung senyawa alkaloid, flavonoid, tanin, saponin, dan terpenoid. Hasil persentase inhibisi hemolisis didapatkan pada 50, 100, 150, 500, dan 1000 ppm, yaitu 14,73%, 24,60%, 38,86%, 43,11%, 50,39%. Simpulan penelitian ini ialah ekstrak sabut kelapa (Cocos nucifera L.) memiliki aktivitas anti-inflamasi. Konsentrasi ekstrak 1000 ppm memiliki aktivitas anti inflamasi tertinggi.\nKata kunci: inflamasi; ekstrak sabut kelapa; stabilisasi membran sel darah merah","PeriodicalId":395652,"journal":{"name":"e-GiGi","volume":"93 8","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"e-GiGi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35790/eg.v13i1.55338","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract: Dental and oral care often causes injuries that can trigger an inflammatory reaction associated with feeling of discomfort. Drugs that can be given for the treatment of inflammation, namely corticosteroids of the glucocorticoid group and non-steroidal anti-inflammatories (NSAIDs). However, these drugs have side effects, therefore, alternatives with minimal toxicity that can be found in plants are preferrable. Coconut coir has the potential to be an anti-inflammatory drug because it contains flavonoids, tannins and saponins. This study aimed to determine the anti-inflammatory activity of coconut coir extract at concentrations of 50, 100, 150, 500, and 1000 ppm. This was a pure experimental study with a post-test-only control design using blood of rats weighing above 250 grams taken through the retro-orbital sinus. The samples were divided into seven groups, namely 50, 100, 150, 500, 1000 ppm, positive control, and negative control. The results showed that coconut coir extract contained alkaloids, flavonoids, tannins, saponins, and terpenoids. The percentages of inhibition of hemolysis were obtained at 50, 100, 150, 500, and 1000 ppm, namely 14.73%, 24.60%, 38.86%, 43.11%, and 50.39%. In conclusion, coconut coir extract (Cocos nucifera L.) has anti-inflammatory activity. Extract concentration of 1000 ppm has the highest anti-inflammatory activity. Keywords: inflammation; coconut coir extract; stabilization of red blood cell membranes Abstrak: Perawatan gigi dan mulut tidak jarang menimbulkan perlukaan yang dapat memicu reaksi inflamasi disertai rasa tidak nyaman. Golongan obat yang dapat diberikan untuk pengobatan inflamasi, yaitu kortikosteroid golongan glukokortikoid dan anti-inflamasi non-steroid (AINS). Namun, obat-obat tersebut memiliki efek samping sehingga dibutuhkan alternatif dengan toksisitas minimal yang dapat ditemukan pada tanaman. Sabut kelapa berpotensi menjadi obat anti-inflamasi karena mengandung flavonoid, tanin, dan saponin. Penelitian ini bertujuan untuk mengetahui aktivitas anti-inflamasi ekstrak sabut kelapa konsentrasi 50, 100, 150, 500, dan 1000 ppm. Penelitian ini merupakan eksperimental murni dengan post-test only control design menggunakan darah tikus yang diambil melalui sinus retro-orbitalis. Kriteria tikus ialah berat di atas 250 gram, yang dibagi menjadi tujuh kelompok, yaitu 50, 100, 150, 500, 1000 ppm, kontrol positif, dan kontrol negatif. Hasil penelitian mendapatkan bahwa ekstrak sabut kelapa mengandung senyawa alkaloid, flavonoid, tanin, saponin, dan terpenoid. Hasil persentase inhibisi hemolisis didapatkan pada 50, 100, 150, 500, dan 1000 ppm, yaitu 14,73%, 24,60%, 38,86%, 43,11%, 50,39%. Simpulan penelitian ini ialah ekstrak sabut kelapa (Cocos nucifera L.) memiliki aktivitas anti-inflamasi. Konsentrasi ekstrak 1000 ppm memiliki aktivitas anti inflamasi tertinggi. Kata kunci: inflamasi; ekstrak sabut kelapa; stabilisasi membran sel darah merah

查看原文本刊更多论文

红细胞膜稳定法测试椰糠提取物（Cocos nucifera L.）的抗炎活性

摘要：牙科和口腔护理经常会造成损伤，从而引发与不适感相关的炎症反应。治疗炎症的药物有糖皮质激素类皮质激素和非甾体抗炎药（NSAIDs）。然而，这些药物都有副作用，因此，最好能从植物中找到毒性最小的替代品。椰糠含有类黄酮、单宁酸和皂苷，因此有可能成为一种消炎药。本研究旨在确定椰糠提取物在 50、100、150、500 和 1000 ppm 浓度下的消炎活性。这是一项纯粹的实验研究，采用只进行试验后对照的设计，通过眶后窦采集体重超过 250 克的大鼠血液。样本分为七组，即 50、100、150、500、1000 ppm、阳性对照组和阴性对照组。结果表明，椰糠提取物中含有生物碱、黄酮类、单宁、皂苷和萜类化合物。在 50、100、150、500 和 1000 ppm 时，溶血抑制率分别为 14.73%、24.60%、38.86%、43.11% 和 50.39%。总之，椰糠提取物（Cocos nucifera L.）具有抗炎活性。关键词：炎症；椰棕提取物；稳定红细胞膜 Abstrak：椰糠提取物具有抗炎活性。可用于治疗炎症的肥胖症药物包括类固醇和非类固醇抗炎药（AINS）。因此，肥胖症患者的饮食习惯会影响他们的身体健康，同时，肥胖症患者的饮食习惯也会影响他们的身体健康，因此，肥胖症患者的饮食习惯也会影响他们的身体健康。它还含有黄酮类化合物、鞣质和皂苷。它的含量分别为 50、100、150、500 和 1000 ppm。该研究采用实验设计和后测对照设计，对眶后窦的抗炎能力进行评估。测试标准为 250 克，测试浓度分别为 50、100、150、500、1000 ppm，阳性对照组和阴性对照组。研究表明，柚皮中含有多种生物碱、黄酮、鞣质、皂苷和萜类化合物。在 50、100、150、500 和 1000 ppm 的浓度下，持久性酶抑制率分别为 14.73%、24.60%、38.86%、43.11% 和 50.39%。这说明椰子油具有抗炎作用。浓度为 1000 ppm 的椰子鞣剂具有抗炎作用。关键词：炎症；椰子鞣剂；稳定细胞膜

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

e-GiGi

自引率

0.00%

发文量