Bacillus spp. – A Potential Probiotic

Journal of Scientific Research and Reports Pub Date : 2024-05-22 DOI:10.9734/jsrr/2024/v30i62082

Vandana K V, .. Harinivenugopal, Shashi Kumar C S, Malashree L, S. B. G., R. B

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Abstract

Aim: To determine the probiotic characteristics for selected Bacillus spp. Study Design: The study was aimed for evaluating the in vitro probiotic properties such as acid and bile tolerance, adhesion ability and antibacterial activity of four strains of Bacillus spp. such as Bacillus cereus, Bacillus subtilis, Bacillus tropicus and Bacillus licheniformis. Place and Duration of Study: Department of Dairy Microbiology, Dairy Science College, Hebbal, Bengaluru, Karnataka, India, study conducted between June 2023 to April 2024. Methodology: Probiotic nature was evaluated by determining the percentage survivability of four strains of Bacillus spp. at pH 2.0 and 0.3 % ox bile concentration. Percentage adhesion was determined using xylene hydrocarbon and antibacterial activity of four Bacillus spp. against test organisms like Escherichia coli, Salmonella spp. and Staphylococcus aureus was tested using agar well diffusion method. Results: In this present study four strains of Bacillus spp. that is B. cereus, B. subtilis, B. tropicus and B. licheniformis exhibited good probiotic characteristics such as acid, bile tolerance, adhesion ability and antibacterial activity against test organisms. Among all the strains B. subtilis have exhibited highest survival rate of 88.01 and 74.86 % at pH 2.0 and 0.3% ox bile concentration respectively, whereas B. tropicus showed 14.5% adhesion ability to xylene hydrocarbon. Regarding antibacterial activity B. subtilis exhibited highest inhibitory zone of 7.1, 6.2 and 4.0 mm against E. coli, Salmonella spp. and Stap. aureus respectively. Conclusion: In conclusion, the results of present study suggested that B. subtilis showed good in vitro probiotic characteristics.

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芽孢杆菌--一种潜在的益生菌

目的：确定选定芽孢杆菌属的益生特性。研究设计：研究旨在评估四种芽孢杆菌属菌株（如蜡样芽孢杆菌、枯草芽孢杆菌、滋养芽孢杆菌和地衣芽孢杆菌）的体外益生特性，如耐酸性和耐胆汁性、粘附能力和抗菌活性。研究地点和时间：研究时间：2023 年 6 月至 2024 年 4 月。研究方法：在 pH 值为 2.0 和牛胆汁浓度为 0.3 % 的条件下，通过测定四株芽孢杆菌的存活率来评估益生菌的性质。使用二甲苯碳氢化合物测定粘附百分比，并使用琼脂井扩散法测试四种芽孢杆菌对大肠杆菌、沙门氏菌属和金黄色葡萄球菌等试验生物的抗菌活性。结果：在本研究中，四种芽孢杆菌属菌株，即蜡样芽孢杆菌（B. cereus）、枯草芽孢杆菌（B. subtilis）、滋养芽孢杆菌（B. tropicus）和地衣芽孢杆菌（B. licheniformis）表现出良好的益生特性，如耐酸性、耐胆汁性、粘附能力和对试验生物的抗菌活性。在所有菌株中，枯草芽孢杆菌在 pH 值为 2.0 和牛胆汁浓度为 0.3% 时的存活率最高，分别为 88.01% 和 74.86%，而热带芽孢杆菌对二甲苯碳氢化合物的粘附能力为 14.5%。在抗菌活性方面，枯草芽孢杆菌对大肠杆菌、沙门氏菌属和金黄色葡萄球菌的最大抑菌区分别为 7.1、6.2 和 4.0 毫米。结论总之，本研究结果表明，枯草杆菌具有良好的体外益生特性。

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Journal of Scientific Research and Reports

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