Neuronostatin regulates proliferation and differentiation of rat brown primary preadipocytes

IF 3.5 4区生物学 Q1 Biochemistry, Genetics and Molecular Biology

FEBS Letters Pub Date : 2024-05-25 DOI:10.1002/1873-3468.14934

Małgorzata Krążek, Tatiana Wojciechowicz, Joanna Fiedorowicz, Mathias Z. Strowski, Krzysztof W. Nowak, Marek Skrzypski

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Abstract

Neuronostatin suppresses the differentiation of white preadipocytes. However, the role of neuronostatin in brown adipose tissue remains elusive. Therefore, we investigated the impact of neuronostatin on the proliferation and differentiation of isolated rat brown preadipocytes. We report that neuronostatin and its receptor (GPR107) are synthesized in brown preadipocytes and brown adipose tissue. Furthermore, neuronostatin promotes the replication of brown preadipocytes via the AKT pathway. Notably, neuronostatin suppresses the expression of markers associated with brown adipogenesis (PGC-1α, PPARγ, PRDM16, and UCP1) and reduces cellular mitochondria content. Moreover, neuronostatin impedes the differentiation of preadipocytes by activating the JNK signaling pathway. These effects were not mimicked by somatostatin. Our results suggest that neuronostatin is involved in regulating brown adipogenesis.

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神经生长抑素调节大鼠棕色原发性前脂肪细胞的增殖和分化

神经生长抑素能抑制白色前脂肪细胞的分化。然而，神经生长抑素在棕色脂肪组织中的作用仍然难以捉摸。因此，我们研究了神经生长抑素对离体大鼠棕色前脂肪细胞增殖和分化的影响。我们报告说，棕色前脂肪细胞和棕色脂肪组织中合成神经生长抑素及其受体（GPR107）。此外，神经生长抑素通过 AKT 途径促进棕色前脂肪细胞的复制。值得注意的是，神经生长抑素会抑制棕色脂肪生成相关标记物（PGC-1α、PPARγ、PRDM16 和 UCP1）的表达，并降低细胞线粒体的含量。此外，神经生长抑素还能通过激活 JNK 信号通路阻碍前脂肪细胞的分化。索马他汀不能模拟这些效应。我们的研究结果表明，神经生长抑素参与调控棕色脂肪的生成。

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来源期刊

FEBS Letters 生物-生化与分子生物学

CiteScore

7.00

自引率

2.90%

发文量

303

审稿时长

1.0 months

期刊介绍： FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.

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