Development of a DNA-Based Lateral Flow Strip Membrane Assay for Rapid Screening and Genotyping of Six High-Incidence STD Pathogens.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Gunho Choi, Keum-Soo Song, Satish Balasaheb Nimse, Taisun Kim
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Abstract

Sexually transmitted diseases (STDs) are a global concern because approximately 1 million new cases emerge daily. Most STDs are curable, but if left untreated, they can cause severe long-term health implications, including infertility and even death. Therefore, a test enabling rapid and accurate screening and genotyping of STD pathogens is highly awaited. Herein, we present the development of the DNA-based 6STD Genotyping 9G Membrane test, a lateral flow strip membrane assay, for the detection and genotyping of six STD pathogens, including Trichomonas vaginalis, Ureaplasma urealyticum, Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma hominis, and Mycoplasma genitalium. Here, we developed a multiplex PCR primer set that allows PCR amplification of genomic materials for these six STD pathogens. We also developed the six ssDNA probes that allow highly efficient detection of the six STD pathogens. The 6STD Genotyping 9G Membrane test lets us obtain the final detection and genotyping results in less than 30 m after PCR at 25 °C. The accuracy of the 6STD Genotyping 9G membrane test in STD genotyping was confirmed by its 100% concordance with the sequencing results of 120 clinical samples. Therefore, the 6STD Genotyping 9G Membrane test emerges as a promising diagnostic tool for precise STD genotyping, facilitating informed decision-making in clinical practice.

开发基于 DNA 的侧流带膜测定法,用于快速筛查六种高发性病病原体并进行基因分型。
性传播疾病(STD)是一个全球关注的问题,因为每天大约有 100 万新病例出现。大多数性传播疾病是可以治愈的,但如果不及时治疗,就会对健康造成严重的长期影响,包括不育甚至死亡。因此,人们非常期待一种能快速准确筛查性病病原体并进行基因分型的检测方法。在此,我们介绍了基于 DNA 的 6STD 基因分型 9G 膜检测试剂盒的开发情况,这是一种侧流条带膜检测试剂盒,用于检测和基因分型六种性传播疾病病原体,包括阴道毛滴虫、尿解脲原体、淋病奈瑟菌、沙眼衣原体、人型支原体和生殖器支原体。在此,我们开发了一套多重 PCR 引物,可对这六种性传播疾病病原体的基因组材料进行 PCR 扩增。我们还开发了六种 ssDNA 探针,可高效检测这六种性病病原体。6STD Genotyping 9G Membrane 检测试剂盒可让我们在 25 °C 下进行 PCR 扩增后,在不到 30 米的时间内获得最终的检测和基因分型结果。6STD 基因分型 9G 膜检验法与 120 份临床样本的测序结果 100%吻合,这证实了它在性病基因分型方面的准确性。因此,6STD 基因分型 9G 膜检验是一种很有前途的诊断工具,可用于精确的性传播疾病基因分型,有助于临床实践中的知情决策。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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