Occurrence and genetic characterization of Enterocytozoon bieneusi in pet dogs in Yunnan Province, China.

IF 2.3 2区医学 Q2 PARASITOLOGY

Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-24 DOI:10.1051/parasite/2024025

Jinhua Jian, Jinrong Zi, Yaxue Wang, Yaming Yang, Yaxing Su, Lan Yao, Benfu Li, Xiaoxue Peng, Jianping Cao, Yujuan Shen, Aiqin Liu

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引用次数: 0

Abstract

Enterocytozoon bieneusi is the most common microsporidian species in humans and can affect over 200 animal species. Considering possible increasing risk of human E. bieneusi infection due to close contact with pet dogs and identification of zoonotic E. bieneusi genotypes, 589 fresh fecal specimens of pet dogs were collected from Yunnan Province, China to determine the occurrence of E. bieneusi, characterize dog-derived E. bieneusi isolates, and assess their zoonotic potential at the genotype level. Enterocytozoon bieneusi was identified and genotyped by PCR and sequencing of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. Twenty-nine specimens (4.9%) were positive. A statistical difference was observed in occurrence rates of E. bieneusi in pet dogs among 11 sampling sites by Fisher's exact test. Fifteen genotypes were identified and all of them phylogenetically belonged to zoonotic group 1, including four known genotypes (EbpC, D, Peru 8, and Henan-III) and 11 novel genotypes. Genotype Henan-III was reported in dogs for the first time. The finding of known genotypes found previously in humans and novel genotypes falling into zoonotic group 1 indicates that dogs may play a role in the transmission of E. bieneusi to humans in the investigated areas.

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中国云南省宠物狗肠虫病的发生和遗传特征。

生物肠孢子虫是人类最常见的微孢子虫，可影响 200 多种动物。考虑到与宠物狗的密切接触可能会增加人类感染生物肠杆菌的风险，以及鉴定生物肠杆菌的人畜共患基因型，研究人员从中国云南省采集了 589 份宠物狗的新鲜粪便标本，以确定生物肠杆菌的发生情况，描述狗源性生物肠杆菌分离株的特征，并在基因型水平上评估其人畜共患的可能性。通过聚合酶链式反应（PCR）和核糖体 RNA（rRNA）基因内部转录间隔区（ITS）测序，对生物肠杆菌进行了鉴定和基因分型。29 份标本（4.9%）呈阳性。通过费舍尔精确检验，观察到 11 个采样点的宠物狗中 E. bieneusi 的发生率存在统计学差异。共鉴定出 15 个基因型，它们在系统发育上都属于人畜共患病第 1 组，包括 4 个已知基因型（EbpC、D、秘鲁 8 型和河南-III 型）和 11 个新基因型。河南-III基因型是首次在狗身上发现。此前在人类中发现的已知基因型和属于人畜共患病第1组的新型基因型表明，在调查地区，狗可能在将E. bieneusi传播给人类的过程中起到了一定的作用。

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来源期刊

Parasite 医学-寄生虫学

CiteScore

5.50

自引率

6.90%

发文量

审稿时长

3 months

期刊介绍： Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.