Effects of asiaticoside on the model of gestational diabetes mellitus in HTR-8/svneo cells via PI3K/AKT pathway.

IF 0.9 4区医学 Q4 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Pub Date : 2024-12-01 Epub Date: 2024-05-24 DOI:10.1080/01443615.2024.2350761

Zhilan Hu, Ya Long, Xiangyue Li, Zhiqin Jia, Mingyan Wang, Xuemei Huang, Xiaolan Yu

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引用次数: 0

Abstract

Background: Asiaticoside (AS) has been reported to improve the changes induced by high glucose stimulation, and it may have potential therapeutic effects on gestational diabetes mellitus (GDM). This study aims to explore the effect of AS on the cell model of GDM and the action mechanism of the PI3K/AKT pathway.

Methods: The GDM model was established in HTR-8/Svneo cells with a high glucose (HG) medium. After the cytotoxicity assay of AS, cells were divided into the control group, HG group and HG + AS group to conduct control experiment in cells. The cell proliferation and migration were detected by CCK-8 assay and scratch test, respectively. The mRNA levels of PI3K, AKT2, mTORC1, and GLUT4 in PI3K/AKT signalling pathway were measured by RT-PCR, and the protein expressions of these signalling molecules were monitored by western blot.

Results: AS showed a promotion effect on the cell proliferation rate of HTR-8/Svneo cells, and 80 μmol/L AS with a treatment time of 48 h had no cytotoxicity. The cell proliferation rate, migration rate, mRNA levels and protein expressions of PI3K, AKT2, mTORC1, and GLUT4 in the HG group were significantly lower than those in the control group, which were significantly increased in the HG + AS group (p < 0.05).

Conclusions: AS can facilitate the cell proliferation and migration in the cell model of GDM, and might play a role in GDM treatment via PI3K/AKT pathway.

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积雪草苷通过 PI3K/AKT 通路对 HTR-8/svneo 细胞妊娠糖尿病模型的影响

背景：据报道，积雪草苷（Asiaticoside，AS）能改善高糖刺激引起的变化，可能对妊娠糖尿病（GDM）有潜在的治疗作用。本研究旨在探讨 AS 对 GDM 细胞模型的影响以及 PI3K/AKT 通路的作用机制：方法：在HTR-8/Svneo细胞中用高糖（HG）培养基建立GDM模型。在 AS 的细胞毒性实验后，将细胞分为对照组、HG 组和 HG + AS 组，进行细胞对照实验。细胞增殖和迁移分别通过 CCK-8 试验和划痕试验进行检测。RT-PCR 检测 PI3K、AKT2、mTORC1 和 GLUT4 的 mRNA 水平，Western 印迹检测这些信号分子的蛋白表达：结果：AS对HTR-8/Svneo细胞的增殖率有促进作用，80 μmol/L AS处理48 h无细胞毒性。HG组的细胞增殖率、迁移率、PI3K、AKT2、mTORC1和GLUT4的mRNA水平和蛋白表达量均显著低于对照组，而HG+AS组的细胞增殖率、迁移率、PI3K、AKT2、mTORC1和GLUT4的mRNA水平和蛋白表达量均显著高于对照组（p 结论：AS对HTR-8/Svneo细胞的增殖和迁移具有促进作用：AS能促进GDM细胞模型中细胞的增殖和迁移，并可能通过PI3K/AKT通路在GDM治疗中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Obstetrics and Gynaecology 医学-妇产科学

CiteScore

2.40

自引率

7.70%

发文量

398

审稿时长

6 months

期刊介绍： Journal of Obstetrics and Gynaecology represents an established forum for the entire field of obstetrics and gynaecology, publishing a broad range of original, peer-reviewed papers, from scientific and clinical research to reviews relevant to practice. It also includes occasional supplements on clinical symposia. The journal is read widely by trainees in our specialty and we acknowledge a major role in education in Obstetrics and Gynaecology. Past and present editors have recognized the difficulties that junior doctors encounter in achieving their first publications and spend time advising authors during their initial attempts at submission. The journal continues to attract a world-wide readership thanks to the emphasis on practical applicability and its excellent record of drawing on an international base of authors.