Expression of free fatty acid receptor 2 in normal and neoplastic tissues

IF 2.8 4区医学 Q2 PATHOLOGY

Experimental and molecular pathology Pub Date : 2024-05-23 DOI:10.1016/j.yexmp.2024.104902

Niklas Ruhnke , Anna-Sophia Liselott Beyer , Daniel Kaemmerer , Jörg Sänger , Stefan Schulz , Amelie Lupp

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引用次数: 0

Abstract

Objective

Little information is available concerning protein expression of the free fatty acid receptor 2 (FFAR2), especially in tumours. Therefore, the aim of the present study was to comprehensively characterise the expression profile of FFAR2 in a large series of human normal and neoplastic tissues using immunohistochemistry thus providing a basis for further in-depth investigations into its potential diagnostic or therapeutic importance.

Methods

We developed a novel rabbit polyclonal anti-FFAR2 antibody, 0524, directed against the C-terminal region of human FFAR2. Antibody specificity was confirmed via Western blot analyses and immunocytochemistry using the FFAR2-expressing cell line BON-1 and FFAR2-specific small interfering RNA as well as native and FFAR2-transfected HEK-293 cells. The antibody was then used for immunohistochemical analyses of various formalin-fixed, paraffin-embedded specimens of normal and neoplastic human tissues.

Results

In normal tissues, FFAR2 was mainly present in distinct cell populations of the cerebral cortex, follicular cells and C cells of the thyroid, cardiomyocytes of the heart, bronchial epithelia and glands, hepatocytes and bile duct epithelia of the liver, gall bladder epithelium, exocrine and β-cells of the endocrine pancreas, glomerular mesangial cells and podocytes as well as collecting ducts of the kidney, intestinal mucosa (particularly enteroendocrine cells), prostate epithelium, seminiferous tubules of the testicles, and placental syncytiotrophoblasts. In neoplastic tissues, FFAR2 was particularly prevalent in papillary thyroid carcinomas, parathyroid adenomas, and gastric, colon, pancreatic, hepatocellular, cholangiocellular, urinary bladder, breast, cervical, and ovarian carcinomas.

Conclusions

We generated and characterised a novel rabbit polyclonal anti-human FFAR2 antibody that is well-suited for visualising FFAR2 expression in human routine pathology tissues. This antibody is also suitable for Western blot and immunocytochemistry experiments. To our knowledge, this antibody enabled the first broad FFAR2 protein expression profile in various normal and neoplastic human tissues.

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游离脂肪酸受体 2 在正常组织和肿瘤组织中的表达

目的有关游离脂肪酸受体 2 (FFAR2) 蛋白表达的信息很少，尤其是在肿瘤中的表达。因此，本研究旨在利用免疫组织化学方法全面描述 FFAR2 在大量人体正常组织和肿瘤组织中的表达情况，从而为进一步深入研究其潜在的诊断或治疗意义奠定基础。方法我们开发了一种新型兔多克隆抗 FFAR2 抗体 0524，该抗体针对人类 FFAR2 的 C 端区域。通过使用 FFAR2 表达细胞系 BON-1、FFAR2 特异性小干扰 RNA 以及原生细胞和 FFAR2 转染的 HEK-293 细胞进行 Western 印迹分析和免疫细胞化学分析，确认了抗体的特异性。然后用该抗体对各种福尔马林固定、石蜡包埋的正常和肿瘤人体组织标本进行免疫组化分析。结果在正常组织中，FFAR2 主要存在于大脑皮层的不同细胞群、甲状腺滤泡细胞和 C 细胞、心脏的心肌细胞、支气管上皮和腺体、肝脏的肝细胞和胆管上皮、胆囊上皮、内分泌胰腺的外分泌细胞和 β 细胞、肾小球系膜细胞和荚膜细胞以及肾脏的集合管、肠粘膜（尤其是肠内分泌细胞）、前列腺上皮、睾丸的曲细精管和胎盘合养细胞。在肿瘤组织中，FFAR2 在甲状腺乳头状癌、甲状旁腺腺瘤以及胃癌、结肠癌、胰腺癌、肝癌、胆管癌、膀胱癌、乳腺癌、宫颈癌和卵巢癌中尤为常见。该抗体还适用于 Western 印迹和免疫细胞化学实验。据我们所知，该抗体首次在各种正常和肿瘤性人体组织中建立了广泛的 FFAR2 蛋白表达谱。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental and molecular pathology 医学-病理学

CiteScore

8.90

自引率

0.00%

发文量

审稿时长

11.5 weeks

期刊介绍： Under new editorial leadership, Experimental and Molecular Pathology presents original articles on disease processes in relation to structural and biochemical alterations in mammalian tissues and fluids and on the application of newer techniques of molecular biology to problems of pathology in humans and other animals. The journal also publishes selected interpretive synthesis reviews by bench level investigators working at the "cutting edge" of contemporary research in pathology. In addition, special thematic issues present original research reports that unravel some of Nature''s most jealously guarded secrets on the pathologic basis of disease. Research Areas include: Stem cells; Neoangiogenesis; Molecular diagnostics; Polymerase chain reaction; In situ hybridization; DNA sequencing; Cell receptors; Carcinogenesis; Pathobiology of neoplasia; Complex infectious diseases; Transplantation; Cytokines; Flow cytomeric analysis; Inflammation; Cellular injury; Immunology and hypersensitivity; Athersclerosis.