Aryl hydrocarbon receptor gene expression in ankylosing spondylitis and its correlation with interleukin-17, RAR-related orphan receptor gamma t expression, and disease activity indices.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-06-14 eCollection Date: 2024-03-01 DOI:10.46497/ArchRheumatol.2023.10203

Maryam Ahmadi, Narjes Soleimanifar, Abdolrahman Rostamian, Maryam Sadr, Hanieh Mojtahedi, Abeda Mazari, Mohammad Hossein Nicknam, Sara Assadiasl

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引用次数: 0

Abstract

Objectives: Considering the role of T helper (Th)17 cells in the pathogenesis of ankylosing spondylitis (AS), the aim of this study was to determine the correlation between aryl hydrocarbon receptor (AHR) gene expression and the expression of Th17-related genes including interleukin (IL)-17 and RAR-related orphan receptor gamma t (RORγt) transcription factor.

Patients and methods: Thirty patients with AS (26 males, 4 females; mean age: 36.1±8.1 years) and 30 age- and sex-matched healthy individuals (26 males, 4 females; mean age: 36.2±14.6 years) were recruited for the case-control study between June 2021 and January 2022. Ribonucleic acid (RNA) was extracted from peripheral blood cells and expression levels of AHR, IL-17, RORγt, and AHR repressor (AHRR) genes were evaluated using real-time polymerase chain reaction technique. The serum level of IL-17 was evaluated with enzyme-linked immunosorbent assay.

Results: The results showed a nonsignificant elevation of AHR, IL-17, and RORγt gene expression in the patient group compared to the control. There was a direct correlation between AHR gene expression and IL-17 and RORγt genes and a negative correlation between AHR and AHRR expression. Moreover, AHR gene expression showed a weak correlation with disease activity indices, including Bath Ankylosing Spondylitis Disease Activity Index, Bath Ankylosing Spondylitis Functional Index, Bath Ankylosing Spondylitis Metrology Index, Bath Ankylosing Spondylitis Global Score, and Ankylosing Spondylitis Quality of Life. Moreover, the serum level of IL-17 was higher in AS patients compared to the healthy group (p=0.02).

Conclusion: Upregulated expression of the AHR gene in ankylosing spondylitis and its correlation with IL-17 and ROR-γ t gene expression suggests that it could be a potential diagnostic and therapeutic target for AS.

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强直性脊柱炎中芳基烃受体基因表达及其与白细胞介素-17、RAR 相关孤儿受体 gamma t 表达和疾病活动指数的相关性。

研究目的考虑到T辅助（Th）17细胞在强直性脊柱炎（AS）发病机制中的作用，本研究旨在确定芳基烃受体（AHR）基因表达与Th17相关基因（包括白细胞介素（IL）-17和RAR相关孤儿受体γt（RORγt）转录因子）表达之间的相关性：在2021年6月至2022年1月期间，招募了30名强直性脊柱炎患者（26名男性，4名女性；平均年龄：36.1±8.1岁）和30名年龄和性别匹配的健康人（26名男性，4名女性；平均年龄：36.2±14.6岁）进行病例对照研究。研究人员从外周血细胞中提取了核糖核酸（RNA），并使用实时聚合酶链反应技术评估了AHR、IL-17、RORγt和AHR抑制因子（AHRR）基因的表达水平。用酶联免疫吸附试验评估了血清中 IL-17 的水平：结果：结果显示，与对照组相比，患者组的 AHR、IL-17 和 RORγt 基因表达均无明显升高。AHR 基因表达与 IL-17 和 RORγt 基因表达直接相关，而 AHR 与 AHRR 表达呈负相关。此外，AHR基因表达与疾病活动指数（包括巴斯强直性脊柱炎疾病活动指数、巴斯强直性脊柱炎功能指数、巴斯强直性脊柱炎计量指数、巴斯强直性脊柱炎总体评分和强直性脊柱炎生活质量）呈弱相关性。此外，与健康组相比，强直性脊柱炎患者血清中的IL-17水平更高（P=0.02）：AHR基因在强直性脊柱炎中的上调表达及其与IL-17和ROR-γ t基因表达的相关性表明，它可能是强直性脊柱炎的潜在诊断和治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.